Re: Freshen up Schiffs

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From:Mick Rentsch <> (by way of histonet)
To:histonet <>
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Dear Bonnie,
you might consider adding Sodium Bisulphite (Metabisulphate) to the schiff
rgt. This would be best if dissolved in a small amount of distilled water
first. This would only freshen up your reagent if excessive exposure to air
had produced oxidation.
You might consider putting your reagent in smaller aliquots with a minimum
air space, eg. blood collection tubes or 100ml amber pharmacy bottles. You
can expect a shelf life of between 8-12 months if stored in this way and
kept at 4C in the dark.
Regards Mike (Downunder)
-----Original Message-----
From: Yanosy, Bonnie <>
To: HistoNet <>
Date: Saturday, 6 February 1999 9:03
Subject: Freshen up Schiffs

>Hope you all won't mind a stupid question but!! what was that stuff  you
>put in Schiffs to perk it up? You can see that too much DESK is a bad
>thing. Bonnie Yanosy

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