-----Original Message-----
From: HistoNet Server <HistoNet@Pathology.swmed.edu>
To: HistoNet Server <HistoNet@pathology.swmed.edu>
Date: Saturday, February 06, 1999 10:02 PM
Subject: Daily Digest


>
>----------------------------------------------------------------------
>
>Date: 6 Feb 1999 01:30:22 -0600
>From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
>Subject: Re: Polishing JB4 - almost lost an assistant ...
>
>
>Patsy Rueg:  You cannot let hundreds of readers die from
>excessive breath-holding. You write well about all sorts
>of matters, so please give all us 'umble 'istonetters
>know the truth about this incident.
>
>>On Mon, 1 Feb 1999, Patsy Ruegg wrote:
>
>>> ... almost lost an assistant by having him saw gma with
>>> an alcohol bath coolant out in the open.
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 6 Feb 1999 09:00:51 -0600
>From: "Yvan Lindekens" <yvan.lindekens@skynet.be>
>Subject: Microtome Leica/Jung Polycut M, anyone has experience with it?
>
>Hi all,
>
>A second-hand Leica/Jung Polycut M microtome has been offered to me, for...
>well... euch...  let's say ... a price I can afford.
>
>It's a sliding type of microtome, and it should be capable of cutting very
>large specimens (200 x 250 mm) in a thickness range of 1-275 um... (I think
>the 1 um is more some kind of theoretical limit).
>
>It's a very heavy machine, it weights euh... about a ton I think... (didn't
>try to lift it as I had back surgery some months ago).
>
>Anyone has any experience with this monster?
>
>BTW: and this has nothing to do with the above: saw last night a
>Reichert/Jung tissue processor on Ebay (Item # 63830183). Sold "as is",
>price at that time was US$ 50...
>
>Yvan Lindekens.
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 6 Feb 1999 11:01:02 -0600
>From: rschoonh@sph.unc.edu
>Subject: Re: Microtome Leica/Jung Polycut M, anyone has experience with it?
>
>Yvan,
>
>In an other life many moons ago I used to work for Reichert-Jung.  My two
MOST
>favorite instruments were the Polycut E  and the Ultracut E/FC4 (each at an
>extream  end of the sectioning spectrum).  Make sure that you have all of
the
>accessories particularly the Hex wrenches.  The 1 micron setting is not as
>theoretical as you may think, at a workshop I was giving someone brought me
a
>small JB-4 block and as I didn't have all of the parts for the 1140
microtome
>(they had unintentionally been sent to another part of the world) I put the
>block in the Polycut and cut 2 micron sections for the customer.  If I can
be
>of help let me know, it's been a while .....but it is like riding a bike
once
>you learn you never forget.
>
>- -- Begin original message --
>>
>> Hi all,
>>
>> A second-hand Leica/Jung Polycut M microtome has been offered to me,
for...
>> well... euch...  let's say ... a price I can afford.
>>
>> It's a sliding type of microtome, and it should be capable of cutting
very
>> large specimens (200 x 250 mm) in a thickness range of 1-275 um... (I
think
>> the 1 um is more some kind of theoretical limit).
>>
>> It's a very heavy machine, it weights euh... about a ton I think...
(didn't
>> try to lift it as I had back surgery some months ago).
>>
>> Anyone has any experience with this monster?
>>
>> BTW: and this has nothing to do with the above: saw last night a
>> Reichert/Jung tissue processor on Ebay (Item # 63830183). Sold "as is",
>> price at that time was US$ 50...
>>
>> Yvan Lindekens.
>- -- End original message --
>
>best regards,
>Bob
>Robert Schoonhoven
>Laboratory of Molecular Carcinogenesis and Mutagenesis
>Dept. of Environmental Sciences and Engineering
>University of North Carolina
>CB#7400
>Chapel Hill, NC 27599
>Phone
>office 919-966-6343
>   Lab 919-966-6140
>   Fax 919-966-6123
>
>**Suppose you were an idiot... And suppose you were a member of Congress
...
>But I repeat myself.-Mark Twain**
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 6 Feb 1999 11:10:28 -0600
>From: "Barry Rittman" <brittman@mail.db.uth.tmc.edu>
>Subject: RE: C.I. 60760 "kernechtrot"
>
>Peter,
> as noted, Kernechtrot is nuclear fast red and I think is a magnificent
>nucelear stain. However it should be noted that rtere are other dyes such
>as the azo dye Kernechtrot salz B which are also used in histochemistry.
>Barry
>
>At 10:46 AM 2/5/99 -0500, you wrote:
>>Peter,
>>The name I have seen for Kernechtrot is nuclear fast red and is used as a
>>counterstain in
>>reticulum and iron stains.
>>
>>Patricia W. Greer
>>Infectious Disease Pathology
>>Centers for Disease Control
>>pwg1@cdc.gov
>>
>>
>>
>>-----Original Message-----
>>From: vandeplas@aurion.nl [mailto:vandeplas@aurion.nl]
>>Sent: Thursday, February 04, 1999 10:01 PM
>>To: Histonet@Pathology.swmed.edu
>>Subject: C.I. 60760 "kernechtrot"
>>
>>
>>Dear all,
>>
>>Could one of you give me the englisch/american name for the nuclear stain
>>"kernechtrot" (german) or "kernechtrood" as we write in The Netherlands.
We
>>use this counterstain instead of heamatoxylin in our wet-workshops for
>>those sections that contain low amounts of antigen. I only have a german
>>written cataloque of Merck (were I purchased the product a long time ago)
>>The C.I. number of  kernechtrot is 60760. The Merck cataloque number:
>>15939.
>>
>>Thanks for your reply.
>>Peter
>>
>>
>>========================================
>>Peter van de Plas
>>AURION
>>Costerweg 5
>>6702 AA Wageningen
>>The Netherlands
>>phone: (31)-317-497676
>>fax: (31)-317-415955
>>http://www.aurion.nl
>>
>>
>>
>>
>
>
>----------------------------------------------------------------------
>
>Date: 6 Feb 1999 11:30:49 -0600
>From: "Kellar, Eric" <kellarec@MSX.UPMC.EDU>
>Subject: RE: Microtome Leica/Jung Polycut M, anyone has experience with it
?
>
>Yvan,
>
>I used a demo Polycut series M a few years ago. I later talked the boss
into
>investing in the Polycut series E (motor driven) from Leica for whole mount
>(100 to 250 mm) paraffin sectioning. I routinely section whole human
larynx,
>prostate, heart and breast at a minimum of 5-6 um for paraffin. Defying
>gravity was the most challenging aspect of sliding microtomy for me. The
>ribbons from the 30 cm c-profile knife must be lifted upward as opposed to
>the ribbons falling downward on a standard rotary microtome.
>
>As Bob Schoonhoven has mentioned, there are many accessories available to
>adapt to your specific needs (freezing device, microscope carrier, plastic
>object plates, embedding molds, tungsten knives, clamps, tools, etc.).
>
>Maintenance and service have been minimal. It is a bench hog, but was worth
>the investment.
>I would also be glad to share more info with you or visit Leica at :
>
>http://www.leica.com
>
>
>Eric Kellar
>Histology/Immunohistochemistry
>University of Pittsburgh Medical Center
>
> ----------
> From:  Yvan Lindekens [SMTP:yvan.lindekens@skynet.be]
> Sent:  Saturday, February 06, 1999 9:45 AM
> To:  histonet@Pathology.swmed.edu
> Subject:  Microtome Leica/Jung Polycut M, anyone has experience with
>it?
>
> Hi all,
>
> A second-hand Leica/Jung Polycut M microtome has been offered to me,
>for...
> well... euch...  let's say ... a price I can afford.
>
> It's a sliding type of microtome, and it should be capable of
>cutting very
> large specimens (200 x 250 mm) in a thickness range of 1-275 um...
>(I think
> the 1 um is more some kind of theoretical limit).
>
> It's a very heavy machine, it weights euh... about a ton I think...
>(didn't
> try to lift it as I had back surgery some months ago).
>
> Anyone has any experience with this monster?
>
> BTW: and this has nothing to do with the above: saw last night a
> Reichert/Jung tissue processor on Ebay (Item # 63830183). Sold "as
>is",
> price at that time was US$ 50...
>
> Yvan Lindekens.
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 6 Feb 1999 11:31:11 -0600
>From: "Katrina Knott" <knottk@amc.org>
>Subject: layered structures
>
> I have been having problems with sectioning layered structures such as
>stomach and skin.  I read that as paraffin cools it can push the tissue
>together creating folds.  As I section I get fold within the tissue
(usually
>within the epithelium layer) that can not be manipulated on the water bath.
>Does anyone have any suggestions on how to get around this problem, ie.
>change of knife angle, hotter water bath, etc.??
>
>Thanks,
>Katrina
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 6 Feb 1999 12:30:20 -0600
>From: "Katrina Knott" <knottk@amc.org>
>Subject: sectioning
>
>I have been having problems with sectioning layered structures such as
>stomach and skin.  I read that as paraffin cools it can push the tissue
>together creating folds.  As I section I get fold within the tissue
(usually
>within the epithelium layer) that can not be manipulated on the water bath.
>Does anyone have any suggestions on how to get around this problem, ie.
>change of knife angle, hotter water bath, etc.??
>
>Thanks,
>Katrina
>
>
>
>----------------------------------------------------------------------
>
>Date: 6 Feb 1999 14:15:25 -0600
>From: "Jeff Silverman" <peptolab@hamptons.com>
>Subject: Re: sectioning
>
>Katrina- Try a hotter water bath. Jeff Silverman
>
>- ----------
>> From: Katrina Knott <knottk@amc.org>
>> To: histonet <histonet@pathology.swmed.edu>
>> Subject: sectioning
>> Date: Saturday, February 06, 1999 1:17 PM
>>
>> I have been having problems with sectioning layered structures such as
>> stomach and skin.  I read that as paraffin cools it can push the tissue
>> together creating folds.  As I section I get fold within the tissue
>(usually
>> within the epithelium layer) that can not be manipulated on the water
>bath.
>> Does anyone have any suggestions on how to get around this problem, ie.
>> change of knife angle, hotter water bath, etc.??
>>
>> Thanks,
>> Katrina
>>
>
>
>----------------------------------------------------------------------
>
>Date: 6 Feb 1999 20:00:56 -0600
>From: "Tom & Beth Galati" <tgalati@shentel.net>
>Subject: Re: help
>
>
>- -----Original Message-----
>From: HistoNet Server <HistoNet@Pathology.swmed.edu>
>To: Tom & Beth Galati <tgalati@shentel.net>
>Date: Saturday, February 06, 1999 7:43 PM
>
>
>>Please do not send ENCLOSURES!!!!!!
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 6 Feb 1999 21:00:35 -0600
>From: sharon osborn <so4decolores@earthlink.net>
>Subject: Re: Does anyone know?
>
>Scott, ProSource is 1.888.322.9406.  Address is 50818 Canyon Ridge
>Drive, Granger, IN 46530.  Brent Riley is owner.  He was the original
>developer of the AccuEdge for Miles tissue Tek.   We are beginning to
>use the blades.  We were pleased with the demo products he sent and the
>prices are very competitive...sharon osborn
>
>Scott Taft wrote:
>
>> Has anyone heard of a company called Pro Source. They make microtome
>> blades. If you have, would you kindly give me their phone number.
>>
>> Thanks,
>> Scott/Tucson AZ
>>
>> _________________________________________________________
>> DO YOU YAHOO!?
>> Get your free @yahoo.com address at http://mail.yahoo.com
>
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 6 Feb 1999 22:00:38 -0600
>From: "bbracing" <bbracing@silk.net>
>Subject: Katrina Knott  re sectioning
>
>Katrina
>Old technology, but I and all my staff still use it to this day, and it
solves
>the wrinkle problem. Use two water baths.  The first one is a small bowl of
>cold tap water to which we add ethanol to make it about 15 to 20% . This
acts
>like a wetting agent.  The sections are then cut, and laid out on the cold
>water bath for a few seconds, (we like to trim our ribons to size while on
>this bath as the wax is very firm and not sticky) then the trimed sections
are
>transfered to the regular 47C water bath.  As the alcohol evaporates, it
pulls
>the sections flat, leaving them wrinkle free. Alcohol is about the only
>wetting agent that you can use, as it evaporates quickly in the warm water,
>most others wetting agents do not, and will contaminate your bath, destroy
>your adhesive (such as gelatin) and  will cause your sections to come off
the
>slides durring subsequent staining proceedures. If you use to much alcohol
in
>the first bath, your ribons will sink, and/or when you transfer them to the
>hot water bath they will blow apart. If you use to little they will not
>flatten as nicely as one would like. A little expirementation will solve
any
>problems.
>Hope this helps
>Kerry
>Kelowna Gen Hospital
>B.C. Canada
>
>
>
>Here are the messages received yesterday!
>
>