Re: [Histonet] unstaining H&E slides

From:Rene J Buesa

It is very simple: just hydrate your sections and place them in 0.1% hydrochloric acid aq.sol. until they are completely destained.
  Wash them in a mild alkaline solution (to neutralize the effects of the acid), wash thoroughly in water and dist. water and restain them with any new procedure.
  René J. wrote:
  Does anyone have a procedure, with a reference, for unstaining H&E slides? I have seached the internet and looked through my textbooks and have not found a written procedure. I have even consulted the procedure manuel at a local hospital, and they did not have a written procedure. I know that you have to back the slides through the stain rack, omitting the H&E and Eosin, but I'm not sure of the recommended timing in each reagent.

Thanks in advance your help
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