Re: [Histonet] Calretinin

From:Dana Settembre

Are you putting the patient section on the same slide as the positive
If you are, and you have precut, preheated positive control slides
waiting to be used,
there's a posibility that the patient section may be placed on top of
some melted
paraffin coming off the pos. cont., thus not giving the pt. case much
to hang on to.

Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA

>>> "Senn, Amy R"  02/19/08 11:34 AM >>>
Hi Histoland....


We are having some problems with our Calret stains.

Our patient tissue keeps floating off & we are not sure what we're
wrong (if anything!)


We do use charged slides.

We float the tissue out in a separate water bath with pure DI water.

We allow the slides to stand upright for 30 minutes to air dry, and
put them in a 62 degree oven for another 30 minutes.

We are careful not to over-handle the slides at anytime, and we make
sure our hands are clean and lotion free.


Sometimes, for weeks and weeks, the tissue is right where we put it
it comes off the stainer.  Other times, for weeks and weeks, it's
And it's only the patient--the control is where's it's supposed to be.


Is there something we're missing?  Something else we can try??

Thanks so much for all your help!!


Amy S.

Histotech, Histology Laboratory

Holy Spirit Hospital

503 N. 21st Street

Camp Hill, PA  17011


(717) 763-2124

Cell: (724) 494-2237


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