I have used these kits quite extensively and found them to be very good, especially for multiplexed assays with two (or more) antibodies from the same species. I have found that incubating the labeled Fab fragment with the primary antibody for longer than 5 min (as recommended in the Molecular Probes protocol) generally produces better results (I typically incubate for 30 min before adding the blocking Ig). It may also be necessary to increase the molar ratio from 1:3 to 1:6 for some low affinity antibodies to amplify the weak signal. It is my general impression from having used a lot of their products that Molecular Probes makes high quality reagents that work quite well.
Scott Turner
Schering-Plough Biopharma
901 California Ave
Palo Alto, CA 94304
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa Gonzalez
Sent: Wednesday, February 20, 2008 05:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Molecular Probes Zenon labeling kits
Has anyone used the labeling kits (Zenon) from Invitrogen to directly label their abs? How well do they work?
They show fabulous examples online of cell staining, but I was wondering how practical they were for tissue staining and epifluorescence detection.
Thanks,
Melissa
Melissa A. González Edick
R&D, Cell Genesys Inc.
500 Forbes Blvd
South San Francisco, CA 94080
p(650) 266-3168
f (650) 266-3080
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