Re: [Histonet] FISH/ISH on Archival slides

From:"Janice Mahoney"

Acetone removes tape coverfilm.  It should only take a few minutes.
Jan Mahoney
Alegent Health

>>> Angela McNabola  02/04/2008 12:19 PM
Hi all,


I'm a "former" histotech performing FISH on cytology samples
cervical).  I have been given archival slides, 2-7 years old, that
been Pap stained.  As you can imagine, they are not necessarily
samples.  Some are even coverslipped with the "tape" method, and it is
taking days to soak it off.


I have tried a few variations of destaining, but the problem is that
not getting any FISH signals at all.  Basically we are using a 7
centromere or 11q (commercially obtained).  Does anybody have any
insight, thoughts, or ideas on how to get this to work?  







Angela McNabola, MS HT(ASCP)SLS, QIHC

Research Scientist

Ikonisys, Inc.

5 Science Park

New Haven, CT 06511



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