Re: [Histonet] ER control tissue

From:"Joe Nocito"

wouldn't using someone else's tissue kind of invalidate those trying to 
establish ASRs? Just wondering.

Joe
----- Original Message ----- 
From: "Bryan Hewlett" 
To: "Richard Cartun" ; ; 

Sent: Sunday, February 04, 2007 11:33 AM
Subject: Re: [Histonet] ER control tissue


> Hi Rich,
>
> I completely agree with your final sentence.
> However, complete in-house standardization of IHC testing (HER2, EGFr, ER, 
> PR, CD20, CD117, etc.),
> absolutely has to include standardization of the in-house fixation and 
> processing!
> Only once this has been achieved, and the controls in use are prepared in 
> a truly similar manner to the test material,
> will we be able to have a standardized IHC test!
> Anything less means that we will continue to be shooting at a wildly 
> moving target.
>
> Best regards,
> Bryan
>
> ----- Original Message ----- 
> From: "Richard Cartun" 
> To: ; 
> Sent: Sunday, February 04, 2007 11:58 AM
> Subject: Re: [Histonet] ER control tissue
>
>
> As we move forward with standardization in IHC testing (HER2, EGFr, ER, 
> PR, CD20, CD117, etc.), we really need to start thinking about using 
> control tissue (including TMAs) fixed and processed in your own 
> laboratory. Although useful for proficiency testing, results obtained on 
> someone else's tissue will not completely validate your testing.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 545-1596
> (860) 545-0174 Fax
>
>>>>  02/01/07 4:41 PM >>>
>
>
>
>
> Hello all -
>
> Does anyone know of a source to order ER controls for IHC    --  other 
> than
> Pantomics in California?   Ideally  I'm looking for an array that would
> contain a strong (3+), moderate (2+), and low/negative (1+) 
> representation.
>
> Thanks for any help!
>
> Barb Moe
> Gundersen Lutheran Medical Center
> La Crosse WI
>
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