[Histonet] peptide-ab blocking
From: | "Carlos G. Perez-Garcia" |
hi everybody
i need to do a peptide-antibody blocking experiment to check the
specificity of an antibody i made. i wonder if somebody has
experience on that. i suppose that just adding 5-10 times more
peptide than antibody in the solution will be enough right? does
anybody have any protocol or suggestion?
thanks in advance
Carlos
Carlos G. Perez-Garcia, Ph.D.
Carlos G. Perez-Garcia, Ph.D.
Molecular Neurobiology Lab (MNL-O)
The Salk Institute
10010 North Torrey Pines Road
92037 La Jolla, CA
USA
cpgarcia@salk.edu
Fax: 858 558 6207
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