[Histonet] Sentinel node procedure
We use a method modified from Cochran
(March AJSP 30(3); 419-420.2006 (letter)
Our modification is as follows.
10 levels are cut from designated block.
(We use an identifier on the block to highlight the special handling
The depth between each level varies with the thickness of the sections.
I generally try to take a section at around 50-80 micron intervals.
The first sections taken from the block should not resemble the last
sections. (making sure you have gone "deep" enough) All sections are
placed on "plus" slides
H/E on levels 1, 3, 5 and 10
Level 2 one slide for S100 (keep an extra unstained)
Level 4 and 6 for Pan Melanoma Cocktail- Biocare (keep an extra
unstained) Levels 7, 8, 9 remain unstained in case there's a need for
I believe the Cochran method describes using HMB-45 at level 4 and
Mart-1 at level 6
Hope this helps!
Becky Orr CLA, HT(ASCP)QIHC
Assistant Manager, Anatomic Pathology
Evanston Northwestern Healthcare
Date: Fri, 2 Feb 2007 15:44:36 -0800 (PST)
From: MICHELLE SEAGLE
Subject: [Histonet] Sentinel node protocol?
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I was interested in knowing what other hospital labs use for their
sentinel lymph node protocols for breast cases and melanoma cases. I
think our hospital's is a little over kill.
Rutherford Hospital HT (ASCP)
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