[Histonet] caspase 3 and background staining
Hi everyone - I see that there are several successful caspase 3 protocols in
rodents running; my question is - in my control tissues the respiratory
epithelium, and interstitial cells of the lung (they look like histiocytes
microscopically) light up; i am looking primarily at the heart and there is an
acceptable level of background for interpretation (in my opinion anyway :) ). I have
done searches and cannot find if these are normal regions to light up - if so,
why? we are using the active caspase 3 antibody and the Dako envision system.
any references as to the normal caspase 3 positive cells would be great! thank
you!
Cheryl Cross
UTCVM
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>