Re: [Histonet] sucrose sectioning
In a message dated 2/20/2006 1:26:21 PM US Mountain Standard Time,
> I work with a scientist that never seems pleased with my cryosectioning of
> fixed sucrose infiltrated liver.
I'm most familiar with using sucrose as a cryoprotectant / infiltration
medium when doing cryoultramicrotomy and immunolabeling at the EM level. In those
cases, sucrose is normally used at around 2.3M concentration after light
aldehyde fixation, and it usually cuts well at around -90 C (the cryosectioning
kits for ultramicrotomes use liquid nitrogen, and they allow you to cut at very
I never could get sucrose to cut well above about -60 to -65 C. It just
turned to mush and became a sticky, stringy mess.
A normal cryostat w/ independent specimen cooling should get down to about
-50 C, but I'd think you'd be right on the edge of being able to use sucrose.
Unless there's some reason not to use the stuff, maybe you could try O.C.T.
or a similar cryo medium designed to be firm at higher temps (say round -20 to
Robert (Bob) Chiovetti, Ph.D.
The Microscope Works
Arizona's Microscopy Resource
132 North Elster Drive
Tucson, AZ 85710-3212 USA
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