Re: [Histonet] PGP 9.5

From:"John C. Dennis"

Dear Peggy

I've used Chemicon's rabbit polyclonal to good effect.

It was used in the 
sniff trade for a while to mark vomeronasal sensory neurons, and 
processes, in particular.  In double labeled preps, I found 
that, in at least several primate species and in dogs, not all sensory 
neurons in 
either the main olfactory or the vomeronasal epithelia express PGP9.5 in 
any given tissue section.  Or at least at detectable levels.  You'll see some or even most but not all 
neurons in a section.  PGP 9.5 is a house keeping protein and is 
presumably up and down regulated depending on metabolic requirements.

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

On Tue, 21 Feb 2006, Lee & Peggy Wenk wrote:

> Anyone have experience with PGP 9.5 (Protein Gene Product)? It demonstrates
> nerve fibers. One of our pathologists wants to use it on skin biopsies for
> diagnosis of neuropathies.
> We would prefer peroxidase for paraffin sections, rather than FITC or FS.
> Just wondering if people are having better luck with monoclonals or
> polyclonals, and if there is a difference in animal species for the primary
> (we'll be doing this on human skin).
> Also - all the papers we find mention thick sections and using a confocal
> microscope. Anyone doing PGP on 5 um sections and/or with a regular light
> microscope?
> One of my students is doing this as her research project. She's done a lot
> of reading on it, and most papers don't discuss the actual procedure or the
> type of antibody, etc. They are on the research aspect, or the diagnostic
> aspect, not on what histotechs need to do the procedures. Those articles
> that do mention techniques have very different methods (of course), and are
> missing important information needed by histotechs.
> We would appreciate a couple of little hints of what works for "real life
> histotechs", to get us started in the right direction for use in a routine
> clinical immunohistochemistry lab.
> Feel free to contact me directly. Vendors included. I'll summarize and relay
> the info back to Histonet.
> Thanks in advance.
> Peggy A. Wenk, HTL(ASCP)SLS
> William Beaumont Hospital
> Royal Oak, MI 48073
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