RE: [Histonet] H&E staining

From:"James Watson"

Try looking at the AFIP staining manual  "Laboratory methods in
Histotechnology" Uses eosin-Phloxine for 2 minutes then two minutes each
for all the alcohols and xylenes, very consistent , very easy, with
excellent staining.

James Watson HT, ASCP
Facilities Manager of Histology
GNF, Genomics Institute of the Novartis Research Foundation
Room C015

-----Original Message-----
[] On Behalf Of Malcolm
Sent: Monday, February 27, 2006 9:47 AM
To: Massimo; eileen dusek
Subject: RE: [Histonet] H&E staining

Doesn't Hummason's Histotechnique manual have this in it?  Not
attacking, just trying to provide a helpful reference!  
Malcolm L. McCallum
Assistant Professor
Department of Biological Sciences
Texas A&M University Texarkana
2600 Robison Rd.
Texarkana, TX 75501
O: 1-903-233-3134
H: 1-903-791-3843


From: on behalf of Massimo
Sent: Mon 2/27/2006 11:40 AM
To: eileen dusek
Subject: Re: [Histonet] H&E staining

After Eosin:

10 - 15 seconds in distillate water
Quick steps for 50, 75, 95%  ETOH (they remove Eosin)
About 20 - 30 seconds for 100% ETOH ( for each step of two. You could
stop longer in this alcohol than the other ones above)

Pay attention!!! That procedure is coming from me: an amateur
naturalist. I think it would be better for you to lissen the opinions of
the "guru" Histologysts of this ML too!!!

Best Regards,


----- Original Message -----
From: "eileen dusek" 
To: "histonet" 
Sent: Monday, February 27, 2006 5:30 PM
Subject: [Histonet] H&E staining

> Hi Everyone,
>  This is a basic question.
>  How long are tech leaving slides in 95% and 100% ETOH after eosin. I 
> feel we are in too long but would like to get other ideas.  Our slides

> have been very washed out, little to no contrast.
>  I appreciate your help
>  Eileen C. Dusek
>  Edward Hospital
> ---------------------------------
> Yahoo! Mail
> Bring photos to life! New PhotoMail  makes sharing a breeze. 
> _______________________________________________
> Histonet mailing list

Histonet mailing list

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>