[Histonet] Testes - Fixation, Staining, Staging
I read the following topic on the histonet and had some questions. Do you
find that prolonged fixation in Bouins > 24 hrs causes a decrease in
acrosomal staining in the testes?
Michael Safron A.S.,HT(ASCP)
WIL Research Laboratories LLC
419-289-8700 ext: 3040
Ashland, Ohio 44805
Here instructions for HE and PAS after Hotchkiss my Pathologists likes;) We
fix testis in Boiun for 24 hours, then transfer them to 70% ethanol in which
they can be kept as long as you wish. We do not rinse them several times in
70% ethanol. If you wish I can ask him more about satging when he returns
from Christmas vacation.
Hope this helpes you:)
I can send you also pictures of my stainings if you like to see the result.
Department of Discovery Biology
Orion Corporation, ORION PHARMA
Orionintie 1, P.O.Box 65, FIN-02101 Espoo, Finland
Tel. +358 10 429 2607, Fax +358 10 429 2924
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