RE: [Histonet] yeast and bacteria on slides
May I suggest you consider this for starters.
The most likely cause is your water floatation bath. How many are used? Do you meticulously clean each one at the end of each day and fill up with fresh deionised water? Can you identify the water bath or baths(if several were used). Here, operator marks their slides with initials for audit purposes so we know who picked up the section, labelled the slide and so which water bath they were using. Hey, bloody audit coming in handy!! Water baths filled with water and left on for several days prior to use will grow micro-organisms, especially if they have been used and not cleaned. If you can pin point sections from each bath and have spare unstained sections from each bath from the "day of recogning", then i suggest you stain a couple of spares from each with PAS and you may get your answer. It has happened here a couple of times in the past.
Hope this helps.
John, Wrexham, Wales.
From: Melissa Jans [mailto:firstname.lastname@example.org]
Sent: 01 February 2005 01:40
Subject: [Histonet] yeast and bacteria on slides
I need some help!!
We have been experiencing some yeast and bacteria showing up on some of our special stains. It started about 2 weeks ago and seems to be sporadic (one day...no problems, the next day, several stains are affected). The yeast and bacteria (rods) are clumped together all over the slide, on the tissue sections, around the sections and on the slide in areas where there is no tissue. The stains we have seen the contamination are the Gram, PAS/D, PAS and GMS. All of these stains are automated except the Gram, so I do not think the special stainer is the problem.
Here is what I have eliminated as problem areas:
-Processor solutions and paraffin (we cut and stained a block from another institute and had contamination)
-Waterbaths, slide drying boards, water containers (used to fill waterbathes), stainer containers, ice pans (all of the mentioned have been thoroughly bleached)
-Tap water and DI water (all water supplies have been tested by sending a sample to micro...there was no bacteria or yeast detected)
-Stay-on used as a slide adhesive (smeared some on a slide and stained it)
-Bad batch of slides (we have had contamination on two different slide sources (vendors))
Does anybody have any ideas? What am I missing? Any suggestions would be much appreciated.
University of Iowa Hospitals and Clinics
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
Histonet mailing list
Mae'r e-bost hwn ac unrhyw ffeiliau a drosglwyddir gydag ef yn
gyfrinachol ac wedi'u bwriadu ar gyfer pwy bynnag y cyfeirir ef ato neu
atynt. Os ydych wedi ei dderbyn drwy gamgymeriad yna gadewch i'r
rheolwr systemau wybod drwy ddefnyddio'r manylion isod.
Mae cynnwys yr e-bost hwn yn cynrychioli barn y sawl a enwir uchod,
felly nid ydyw'n dilyn ei fod yn cynrychioli barn GIG Gogledd Ddwrain
Cofiwch fod yn ymwybodol ei bod yn bosibl y bydd disgwyl i
Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru roi cyhoeddusrwydd i gynnwys
unrhyw ebost neu ohebiaeth a dderbynnir, yn unol ag amodau’r Ddeddf
Rhyddid Gwybodaeth 2000. I gael mwy o wybodaeth am Ryddid Gwybodaeth,
cofiwch gyfeirio at wefan Ymddiriedolaeth GIG Gogledd Ddwyrain Cymru
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you have received this email in error please notify
the system manager using the details below.
The contents of this email represent the views of the individual(s)
named above and do not necessarily represent the views of the
North East Wales NHS Trust.
Please be aware that, under the terms of the Freedom of Information Act
2000, the North East Wales NHS Trust may be required to make public the
content of any emails or correspondence received. For futher
information on Freedom of Information, please refer to the North East
Wales NHS Trust website at www.newalesnhstrust.org.uk
For further assistance, please contact
Histonet mailing list
<< Previous Message | Next Message >>