[Histonet] RE: aqueous mounting medium
From my experiments with X-gal as chromogen for IHC techniques it
appeared that the blue-greenish reaction product (with X-gal +
ferro/ferricyanide) is very stable and survives any mounting medium.
You may dehydrate and mount organically.
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center M2-230
NL-1105 AZ Amsterdam
phone: +31 20 5665631
fax: +31 20 6960389
----- Original Message -----
From "Amanda MacFarlane"
Date Mon, 31 Jan 2005 11:38:24 -0500 (EST)
Subject [Histonet] aqueous mounting medium
I have stained whole tissues for beta-galactosidase (X-gal) and am now
cryosectioning the tissues. I have been fixing the sections in 4% PFA
overnight at 4 degrees Celsius and cover slipping with 70% glycerol.
Coverslipping with glycerol is a time-consuming pain in the butt so
checked out glycerol vinyl alcohol aqueous (GVA) mounting solution
and Supermount aqueous mounting medium (Biogenex) as alternatives. It
appears that these products have not been tested with beta-gal
so I'm wondering if people use these products and/or what people
Amanda MacFarlane, PhD
Division of Nutritional Sciences
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