[Histonet] Mast cell staining
We do toluidine blue staining regularly. We have found that the biggest
cause for poor staining is the pH of the solution. It should be about
2.5. Our solution is simple: Toluidine blue, 0.5 gm; 20% alcohol, 100
ml. Place slides in T blue solution - 2 min. Rinse in water.
Differentiate in 2 changes each 95% alcohol and absolute alcohol - 1 min
each. Air dry slides. Coverslip. It is important to air dry the
slides as the alcohol left on the slides can continue differentiation.
Results: Mast cells: blue with magenta granules.
Hope this helps. Cheryl
Cheryl Crowder, BA, HTL(ASCP)
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Skip Bertman Drive
Baton Rouge, LA 70803
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