am writting you about MMA embedding
i had a protocol using only MMA with peroxide benzoyle, embedding at 27 C°, tissue was preserved, but immunostainnig was impossible
and we found another protocol using MMA, methylbenzoate, PEG 400, butylmetacrylate, this one preserves tissue for immunostaining,and it's embedded at -20°C
does anyone use the first or the other, wich one is better for light microscopy ? ou do you have other protocols ?
i would like to preserve mineralized tissue, and its interface with a metal biomaterial
any information or advice would be helpfull
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