[Histonet] MMA

From:Myri37@aol.com

hello everyone
am writting you about MMA embedding
i had a protocol using only MMA with peroxide benzoyle, embedding at 27 C, tissue was preserved, but immunostainnig was impossible
and we found another protocol using MMA, methylbenzoate, PEG 400, butylmetacrylate, this one preserves tissue for immunostaining,and it's embedded at -20C 

does anyone use the first or the other, wich one is better for light microscopy ?  ou do you have other protocols ?  
i would like to preserve mineralized tissue, and its interface with a metal biomaterial
any information or advice would be helpfull
Thank you 
Myriam


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