Re: [Histonet] mouse perfusion

From:Geoff McAuliffe

Hi Sharon:

    You may be able to shorten the time of perfusion and avoid hand 
cramps by reducing the amount of washout solution. In my experience many 
people spend way too much time and solution washing blood out of the 
circulatory system. 10 ml of buffer or saline is plenty to wash the 
blood out of a mouse. After that it is on to fixative. You will never 
get all of the blood out of every vessel so why try.


Cooperman, Sharon (NIH/NICHD) wrote:

>Dear Histoneteers,
>I am having trouble with mouse perfusion.  We do an intracardiac (left
>ventricle perfusion).  I start out fine, but about halfway through the
>perfusion I get a bad hand cramp (I have writer's cramp) and can't hold the
>needle well.  I often wind up punching through the septum or the anterior
>wall and the rest of the perfusion doesn't go well, so I get good flushing
>of blood but poor fixation.  Does anyone know of a method for holding the
>needle without hand fatigue?  There are people in my lab who don't have
>writer's cramp who have much less trouble with perfusions, but I can't
>change the writer's cramp stuff.
>Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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