RE: [Histonet] massons trichrome

From:Gayle Callis

The Weigerts may also be old - we made up our Weigerts fresh each time
since the oxidizer continues to do just that, oxidize the hematoxylin until
it no longer stains properly.   This is explained in Sheehan and Hrapchak
book, even though some people use their Weigerts longer, we never took the

At 05:54 PM 2/12/2004 -0500, you wrote:
>Incomplete washing after Weigert's will cause the purple staining. And
>Make sure the Weigert's was correctly made. 
>Rena Fail
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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