immunostaining NMDA receptor proteins

From:"Mary B. Olive"

Hello, all.  Does anyone have any suggestions or tips for immunostaining NMDA receptor subunit (and associated) proteins in 4% parafomaldehyde fixed rat cortex?  We currently use the Vectastain HRP kits (Vector Labs) with DAB as a chromogen.  I am especially interested in hearing about your experiences with different forms of antigen retrieval (esp. autoclave) or fixation techniques/times that may improve the signal.
Thanks in advance,
Mary B. Olive
Research Assistant
Pediatric Neurology
Children's Hospital of Michigan
Wayne State University

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