RE: Objective cleaning.

From:Gary Gill

Regardless of the solvent used, never put it directly onto a lens surface, as it may seep around the lens circumference and loosen the cement that holds the lens in place.  Instead, first put a drop or two of cleaner on a double thickness of lens tissue and then clean the lens.  For recessed concave surfaces of plano lenses, use a sharpened bamboo stick to press on the moistened lens tissue and move it over the lens surface.  Move to a dry area of lens tissue, and thoroughly dry the residual fluid to avoid spotting that can make the lens dirtier than it was initially.  Use an inverted eyepiece as a jeweler's-like loupe to inspect the lens before cleaning, so you'll know what you're dealing with, and after cleaning to confirm that you've accomplished what you intended.
 
Gary Gill
-----Original Message-----
From: Ian Montgomery [mailto:ian.montgomery@bio.gla.ac.uk]
Sent: Wednesday, February 26, 2003 9:33 AM
To: histonet@pathology.swmed.edu
Subject: Objective cleaning.

        Many thanks for the information and suggestions. Yesterday afternoon I searched my books and last night trawled through the internet searching for information. Savile Bradbury in his Introduction to the Optical Microscope recommends absolute alcohol. Ether, mixtures of ether and alcohol, xylene, various proprietary cleaners and isopropyl alcohol (propan-2-ol) are all used. I'm swaying towards the isopropyl alcohol but before I commit myself I'll contact Zeiss. Anyone had any experience with isopropyl alcohol?
Ian.

Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery@bio.gla.ac.uk


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