Hi All

Does anyone have experience staining rat intestine with methylene blue to look for aberrent dysplastic crypts. The mucosal side is examined directly under a dissecting microscope, the method is that of RP Bird from Cancer Letters 37 (1987) 147-151

I am having difficulty differentiating the crypts as everything stains intensely

Best Wishes

Mike Bromley

Chief Biomedical Scientist
Pathology
Dumfries & Galloway Royal Infirmary

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