RE: Methyl green-Saffrain-o
|From:||Mass Histology Service |
Here's our modified procedure:
slides to water
routine hematoxylin, differentiate, blue, etc.
0.02% fast green for 5 minutes
1.0% glacial acetic acid, 3 dips
0.1% saf O, 10 minutes
James E. Staruk, HT(ASCP)
Mass Histology Service
(A division of DVMLab, Inc.)
From: CYNTHIA HAYNES [mailto:email@example.com]
Sent: Tuesday, February 25, 2003 11:29 AM
Subject: Methyl green-Saffrain-o
Hello my fellow histonetters,
Does any one have a protocol for Fast green(methyl green)-Saffrain-o
stain for paraffin on decaled bone. The procedure I am using is not working
for some strange reason. If any has a procedure for making up the solutions
I would greatly appreciated.
Thanks in Advance
Cynthia Haynes H.T.
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