I am trying to stain all blood vessels in the brainstem of the rat. 
Ideally I would like to differentiate arteries and veins. I have 
attempted what is known as a double ink perfusion, using blue ink then 
red ink, but due to the limited information I have been able to gain on 
this method, I have not had satisfying results so far. Can anyone give 
me a detailed description of this technique or of any other methods 
which may be used to label arteries and veins, that does not involve 
using immunohistochemistry.


Dr Dina Lonergan
Department of Physiology
University of Bristol

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