Phospholipid Staining


I am looking for a staining technique for phospholipids.  We have
tissue, unfixed, frozen that is suspect of phospholipidosis.  I would
like to run some type of quanitative stain to demonstrate the

One procedure I am looking at is  Sudan Black B Method for
Phospholipids (Elftman 1957)has anybody ever tried this method?

Michael A. Safron A. S., HT(ASCP)
Manager, Histology
WIL Research Laboratories Inc.

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