I am looking for a staining technique for phospholipids. We have tissue, unfixed, frozen that is suspect of phospholipidosis. I would like to run some type of quanitative stain to demonstrate the increase. One procedure I am looking at is Sudan Black B Method for Phospholipids (Elftman 1957)has anybody ever tried this method? Michael A. Safron A. S., HT(ASCP) Manager, Histology WIL Research Laboratories Inc.