RE: dividing cell preps

From:Gary Gill


The product is Mount-Quick (mounting and tissue transfer medium [MOUNT-QUICK
Xylene Soluble Mounting Medium, 30cc tube Packaging allows maximum control
during coverslipping. Fast drying. Clear. UNIQUE AS A TISSUE TRANSFER
MEDIUM. Every lab needs at least one for emergencies.   each 6270A $12.00
(6+ tubes = H)]), available from Newcomer Supply

Brown GG, Tao LC.  Restoration of broken cytology slides and creation of
multiple slides from a single smear preparation.  

Acta Cytol 1992 Mar-Apr;36(2):259-63.

A technique was developed for restoring broken cytology slides so that they
are close to their original condition and for making multiple slides from a
single smear preparation. The method is applicable to both cytologic
preparations and histologic sections. In this study the fragmented smear
preparation was treated with Pro-Texx, which penetrated, impregnated and
solidified the full thickness of the pieces of the smear, enabling them to
be lifted from the pieces of the broken slide. The removed pieces of the
smear preparation were reassembled onto a new slide, which was then
restained and coverslipped. In preparing multiple teaching slides, the
treated smear preparation was divided as planned, with each portion mounted
onto a separate slide, which was then restained and coverslipped. Ten other
fine needle aspiration cases with broken slides have been restored, and more
teaching slides were prepared from a single smear preparation using the same
technique. All were equally successful. This technique provides an excellent
method of smear transfer in cases of broken slides and creation of multiple
slides from a single smear preparation for cytology teaching. This is
particularly useful for unusual cases.

Sherman ME, Jimenez-Joseph D, Gangi MD, Rojas-Corona RR.  Immunostaining of
small cytologic specimens. Facilitation with cell transfer.  Acta Cytol 1994

Immunocytochemical study of cytologic specimens is often limited by the
number of slides containing diagnostic cells. This study examined the
effectiveness of transferring cells from a single smear to multiple slides
in order to perform a battery of immunocytochemical stains on limited
material. Immunostaining performed on four effusions and five fine needle
aspirates yielded the expected results for most of the antibodies commonly
employed in diagnostic pathology. Background staining was generally low
following cell transfer, and the morphology of the cells was preserved.
These results suggest that cell transfer in combination with
immunocytochemistry may prove useful in the cytologic diagnosis of malignant
lymphoma, neuroendocrine neoplasms, prostatic and mammary adenocarcinoma,
and other malignant tumors.

The technique works well with some practice.

Gary Gill

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