Boric Acid Buffer for BudR staining
We do a lot of BudR staining in our lab.
This is our recipe for Boric Acid Buffer :-
Boric Acid Buffer
Stock A: 0.2 molar Orthoboric Acid solution (7.44g in 600ml distilled
Stock B: 0.05 molar Sodium Tetraborate(Borax) solution (9.5g in 500ml
Mix 550ml of stock A and 450ml of stock B; pH 8.4.
Hope this helps. Our protocol is very robust and we haven't had any
problems with it. If anyone wants to see our protocol, please let me know.
<< Previous Message | Next Message >>