"Dear David,
Considering the the use of HCl etc. you obtained an older BrdU IHC staining
protocol. Here we once tried to stain BrdU containing FFPE sections just by
using a standard antigen retrieval method (citrate pH6.0). It worked
perfectly fine!"

Chris, Interesting! I tried this last week and, altho'  I got positive
nuclei, the number of positives was far higher when I used the traditional
HCL/ trypsin method. Did you compare the two?
Carl
Carl Hobbs
Laboratory Manager
Molecular Neurobiology Group
MRC Centre For Developmental Neurobiology
New Hunts House
Guys Campus
kings College London
SE1 1UL
020 7848 6810/fax 6816