It might depend on what is going to be done with the sections after they
come out of storage. The best thing would be for you to do the experiment
yourself, store some sections from the same brain both ways and see how they
perform in subsequent proceedures. Then you will know exactly how changes in
storgage impact your tissue in your lab.

fsapr wrote:

> Hello. I am trying to find out the effects of storing 200 micrometer
> hippocampal slices in phosphate buffer ( instead of PBS ) at 4 C. The slices
> were fixed in 4% paraformaldehyde for 3 hours. Thank you.
>
> Austin Ross
>  University of Alaska Fairbanks
> P.O. Box 750392
> Fairbanks, AK 99775
> School: (907)-474-2656
> Home: (907)-457-2027
> fsapr@uaf.edu

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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