We use Nuclear Fast Red.

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792-2472
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: Gayle Callis [mailto:uvsgc@montana.edu]
Sent: Monday, February 25, 2002 11:29 AM
To: histonet@pathology.swmed.edu
Subject: counterstaining with ISH


Are people counterstaining sections after ISH using nonradioactive method
with BCIP/NBT chromogen?  If so what do you prefer?


Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)