RE: counterstaining with ISH

From:"Sebree Linda A."

We use Nuclear Fast Red.

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792-2472
FAX: (608)262-7174

-----Original Message-----
From: Gayle Callis []
Sent: Monday, February 25, 2002 11:29 AM
Subject: counterstaining with ISH

Are people counterstaining sections after ISH using nonradioactive method
with BCIP/NBT chromogen?  If so what do you prefer?

Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)

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