I like the contrast of nuclear fast red Gayle, its easy, photographs well,
doesn't fade, and can be mounted with synthetic mountant like the BCIP/NBT.

regards,
rob

-----Original Message-----
From: Gayle Callis [mailto:uvsgc@montana.edu]
Sent: Monday, February 25, 2002 11:29 AM
To: histonet@pathology.swmed.edu
Subject: counterstaining with ISH


Are people counterstaining sections after ISH using nonradioactive method
with BCIP/NBT chromogen?  If so what do you prefer?


Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)



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