Hi everybody

Some time ago I used this antibody with great plessure on rat tissues. As
antigen-retrievel I used citrate-buffer (pH 6.0) for 15 minutes, beautifull
staining and hardly any background. But on one day it did not work anymore. 
Trying and trying but no succes. At last I used a adjustable pressure cooker
at a temp of about 121 degrees for 3 minutes. It took some time more but I
had my staining back. 
Is anyone familiar with this strange story?
And is it possible to use a normal pressure coocker, in stead of a
adjustable pressure cooker?


Best regards

Joost Bruijntjes
TNO Nutrition and Food Research
Zeist
Holland
Phone + 31 30 6944483
                         6944480
E-mail J.Bruyntjes@voeding.tno.nl