Region VII S/C in Colorado

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Subject:	Region VII S/C in Colorado

Contact John McGinley to register
Location:  Children's Hospital  Denver, Colorado
Colorado Society for Histotechnology and NSH Region VII S/C
Session 1- Friday April 20, 2001
#1a Immunohistochemistry Update - 2001"
Richard Cartun, PhD Hartford Hospital, CT
Case studies will be used to review current applications of IHC to solid
tumor identifications, prognostic markers in breast cancer, cytology, and
infectious disease detection.  Use of molecular pathology methods will also
be discussed. 
#1b "The Animal & Human Prion Diseases  (AKA The TSE'S)" Elizabeth S.
Williams DVM, PhD, Diplomat ACVP University of Wyoming This presentation
will review the human animal transmissible spongiform encephalopathies (CJD,
VCJD, BSE, CWD, TME, etc.); what they are and how we diagnose them.
#1c "The Chemistry of Tissue Processing"
Jerry Fredenburgh PhD,  Richard Allen 
This is a workshop which consists of a discussion of the basic chemistry and
chemical reactions that take place during tissue processing.  Also discussed
are how these chemical reactions influence the results of processing and
staining. A manual and slides will be used as teaching aids. 
#1d "What the Heck is HIPAA and Why Should I Care?"
Jackie Driscoll, MT(ASCP)SM,  Patient Choice
Gain a general understanding of the three main areas of focus of the new
federal regulations: electronic transaction, security and privacy. Learn how
the regulations may affect your work environment and your rights as a
private citizen.
Session 2- Saturday April 21, 2001 AM
#2a "Are You Singing The Blues?"
Gayle Callis, BS, MT, HT, HTL(ASCP) 
Montana State University
This is a hands on hematoxylin and eosin staining workshop using several
commercially made hematoxylin formulations.  The first half will be a brief
discussion of the history. Formulations and chemistry of H&E staining will
be followed by a hands-on portion.  If time allows troubleshooting hints on
problems commonly encountered with the H&E will be presented.
Troubleshooting hints are part of the handout for future reference.  NBF
fixed tissue sections will be supplied but attendees may wish to stain
sections from their labs, a minimum of four slides, even though there are
seven different hematoxylins available for staining.
#2b  "Application of Basic IHC Including Incorporating new Antibodies in
Your Lab"
Denise Sapier, HT, BS   Dako Corporation
This workshop will focus on the basics of Immunohistochemistry to include
discussion on the selection of monoclonal versus polyclonal antibodies in
routine work, and troubleshooting hints on retrieval pretreatment and
background staining.  Participants will also learn how to incorporate a new
antibody or a new procedure into their laboratory routine.
#2c  "Applications of Immunohistochemistry to Diagnostic Hematopathology"
Richard Cartun, PhD Hartford Hospital, CT This lecture will review current
applications of IHC to diagnostic hematopathology.  Case studies will be
used to demonstrate the use of antibodies to CD5, CD10, CD23, ALK-1, Bcl-6,
CyclinD1 and others.  Tissue pretreatments and detection systems will be

Session 3- Saturday April 21, 2001 PM		
#3a "The Her-2/neu Gene and Protein in Breast Cancer: Prognostic, Predictive
and Target of Therapy."
Robert Gasparini, MS, CLSp(CG) Ventana
Over the last 18 months, the question in the testing labs has changed from
"should we test for Her2?" to "How should we test for Her2?" Data on the two
most common methods of testing, IHC and FISH will be discussed in detail.
The Her-2/neu oncogene represents the next generation of "molecular markers"
that have the potential to be utilized for: a) prognostic factor; b)
predictive factor; and c) target of new therapies.
#3b " Organs and the Role of  Fetal Stem Cells " Gail Deutsch, MD
Children's Hospital, Denver There has been tremendous strides in stem cell
research in the past couple of years.  Find out how distinctly different
organs emerge from multipotential precursor cell populations in the
developing murine embryo.  
#3c "Tricks of the Trade" moderated by: Donna Harp, HT(ASCP) Ventana Bring
your problems and bring your solutions in this round table format.
Participants will share their experiences, thoughts and opinions on various
technical issues.

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