Hi Patsy
We routinely do CMV by IHC and if the pathologist doesn't trust the negative
result of IHC, we occasionally do ISH using Novocastra's FITC-labeled probe
directed against an early RNA transcript (NCL-CMV). Detection is by
rb-a-FITC/AP (NCL-ISH-D) and NBT/BCIP. Works reasonably well. When we
introduced ISH for CMV about 3 years ago, I checked the literature which -
at that time - considered IHC and non-radioactive ISH to be about equally
sensitive for CMV. So far we didn't reveal any of the IHC-neg cases to be
ISH-pos.

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland
http://www.pathology.unibe.ch

-----Original Message-----
From: <Patsy.Ruegg@UCHSC.edu>
To: <histonet@pathology.swmed.edu>
Sent: Mon, 19 Feb 2001 18:30
ISH for CMV


> If you are doing CMV by ISH what is the preferred probe kit, which
company,
> ENZO?  I am just curious about how many do CMV by ISH or IHC?
> Patsy Ruegg
>