Re: Frozen section adherence again!!!!!!!

From:"John C. Dennis" <>

Dear Szigeti Csaba

A while back, following the advice of those in Histoland, I began doing

Use ordinary glass slides that you've coated with poly-L-lysine.  You can
buy a poly-L-lysine solution ready to dilute from Sigma.

Make sure your tissue sees no alcohols during processing.

Let the sections air dry for about an hour at room temperature.

Go to town.

Yours for mutual cohesion

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

On Fri, 9 Feb 2001, Szigeti Csaba wrote:

> Please write me  some tips....
> my spinal cord slices goes down from the silan slides during the protokoll
> of in situ hibr.
> I wrote some days ago but not any answer  yet......
> Csaba

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