storage of frozen tissue

From:Renee Thacker <>

I am having trouble sectioning frozen tissue that has previously been 
sectioned.  The tissue will section wonderfully the first time then 
when resectioned weeks/months later the sections rip, separate from 
the embedding media, and sections in general just look awful.

So I was wondering how people are storing frozen tissue that is 
sectioned and then will need to be sectioned at a later date stored 
(liquid nitrogen, -80 freezer wrapped in foil, etc.?). Generally I'm 
working with fresh frozen unfixed tissue but some fixed tissue as 
Thanks any help would be greatly appreciated!
Renee Hoyle-Thacker
CIIT Centers for Health Research
6 Davis Dr.
Research Triangle Park  NC  27709
Voice: (919) 558-1322

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