storage of frozen tissue
|From:||Renee Thacker <RThacker@ciit.org>|
I am having trouble sectioning frozen tissue that has previously been
sectioned. The tissue will section wonderfully the first time then
when resectioned weeks/months later the sections rip, separate from
the embedding media, and sections in general just look awful.
So I was wondering how people are storing frozen tissue that is
sectioned and then will need to be sectioned at a later date stored
(liquid nitrogen, -80 freezer wrapped in foil, etc.?). Generally I'm
working with fresh frozen unfixed tissue but some fixed tissue as
Thanks any help would be greatly appreciated!
CIIT Centers for Health Research
6 Davis Dr.
Research Triangle Park NC 27709
Voice: (919) 558-1322
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