Re: Daily Digest

From:
Denise Bland-Piontek <bland008@umn.edu>
I've always been told that the trick to keeping film coverslips on is to make
sure you clear your slides in three changes of virgin xylenes before
coverslipping. I'm a fan of glass coverslips, but this trick seems to have
worked for another lab I worked in previously.
Denise Bland-Piontek, HTL (ASCP)
University of Minnesota

greg tesdall wrote:

> I have had the Sukura since 1985 and use it for
> histo/cyto. I have never had a coverslip come off. For
> us it's a great machine. >
> ----------------------------------------------------------------------
> >
> > Date: 11 Feb 2001 03:58:01 -0600
> > From: RichardWHorobin@aol.com
> > Subject: Masson on resin section
> >
> >
> >
> > Diane Berenek wrote saying:
> >
> > > I need a procedure for staining Methacrylate
> > sections using Masson
> > > Trichrome. Would it be the same for Paraffin
> > embedded sections, or is there
> > > some modification needed?
> >
> > By methacrylate do you mean the 'water miscible'
> > glycol methacrylate (GMA),
> > or methyl methacrylate (MMA)?
> >
> > If MMA, since the resin is removed prior to
> > staining, there should be no
> > particular problem using a 'designed for paraffin'
> > procedure. I will rephrase
> > that: no EXTRA problems, as Masson's trichrome is an
> > innately complex
> > procedure.
> >
> > If GMA, the resin is not (cannot) be removed prior
> > to staining, and this
> > greatly influences the staining process. Stains
> > involving sizeable dyes -
> > such as aniline blue or light green - will tend to
> > understain their usual (ie
> > 'paraffin section usual') targets, but to give
> > background resin staining
> > which is hard to remove (try alcohol). The acidic
> > phosphomolybdic acid
> > exacerbates this effect. If you find a way around
> > these problems, do please
> > let me know! The simpler trichromes, such as van
> > Gieson's, can be done on GMA
> > material, especially if penetration aids such as
> > alcohol are used to swell
> > the resin.
> >
> > Richard Horobin
> > Institute of Biomedical & Life Sciences, University
> > of Glasgow
> > T direct +44-1796-474 480 --- E
> > RichardWHorobin@aol.com
> > "What should we expect? Everything."
> >
> >
> >
> > ******************* NOTE *******************
> > There may be important message content
> > contained in the following MIME Information.
> > ********************************************
> >
> >
> > - ------------------ MIME Information follows
> > ------------------
> >
> >
> > - --part1_db.101ac806.27b7961f_boundary
> > Content-Type: text/plain; charset="US-ASCII"
> > Content-Transfer-Encoding: 7bit
> >
> > <<<<<< See above "Message Body" >>>>>>
> >
> > - --part1_db.101ac806.27b7961f_boundary
> > Content-Type: text/html; charset="US-ASCII"
> > Content-Transfer-Encoding: 7bit
> >
> > <HTML><FONT FACE=arial,helvetica><FONT  SIZE=2>
> > <BR>Diane Berenek wrote saying:
> > <BR>
> > <BR><BLOCKQUOTE TYPE=CITE style="BORDER-LEFT:
> > #0000ff 2px solid; MARGIN-LEFT:
> > 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px">I need a
> > procedure for staining
> > Methacrylate sections using Masson
> > <BR>Trichrome. Would it be the same for Paraffin
> > embedded sections, or is
> > there
> > <BR>some modification needed?</FONT><FONT
> > COLOR="#000000" SIZE=3
> > FAMILY="SANSSERIF" FACE="Arial"
> > LANG="0"></BLOCKQUOTE>
> > <BR></FONT><FONT  COLOR="#000000" SIZE=2
> > FAMILY="SANSSERIF" FACE="Arial"
> > LANG="0">
> > <BR>By methacrylate do you mean the 'water miscible'
> > glycol methacrylate
> > (GMA),
> > <BR>or methyl methacrylate (MMA)?
> > <BR>
> > <BR>If MMA, since the resin is removed prior to
> > staining, there should be no
> > <BR>particular problem using a 'designed for
> > paraffin' procedure. I will
> > rephrase
> > <BR>that: no EXTRA problems, as Masson's trichrome
> > is an innately complex
> > <BR>procedure.
> > <BR>
> > <BR>If GMA, the resin is not (cannot) be removed
> > prior to staining, and this
> > <BR>greatly influences the staining process. Stains
> > involving sizeable dyes -
> > <BR>such as aniline blue or light green - will tend
> > to understain their usual
> > (ie
> > <BR>'paraffin section usual') targets, but to give
> > background resin staining
> > <BR>which is hard to remove (try alcohol). The
> > acidic phosphomolybdic acid
> > <BR>exacerbates this effect. If you find a way
> > around these problems, do
> > please
> > <BR>let me know! The simpler trichromes, such as van
> > Gieson's, can be done on
> > GMA
> > <BR>material, especially if penetration aids such as
> > alcohol are used to swell
> >
> > <BR>the resin.
> > <BR>
> > <BR>Richard Horobin
> > <BR>Institute of Biomedical & Life Sciences,
> > University of Glasgow
> > <BR><B>T direct +44-1796-474 480 --- E
> >  RichardWHorobin@aol.com</B>
> > <BR><I>"What should we expect?
> > Everything."</I></FONT></HTML>
> >
> > - --part1_db.101ac806.27b7961f_boundary--
> >
> >
> >
> ----------------------------------------------------------------------
> >
> > Date: 11 Feb 2001 14:45:54 -0600
> > From: "Bill Sinai" <bills@icpmr.wsahs.nsw.gov.au>
> > Subject: Re: Daily Digest
> >
> >
> > Dear All,
> > We have had the Sakura Tape Coverslipper for about
> > 10 years now and have
> > approximately 2,000,000 slides which the coverslip
> > and tissue have come
> > away.  This can begin happening as soon as 2 years
> > after coverslipping but
> > most seem to last at least 4-5 years.
> >
> > We sent some slides to Sakura in Japan and their
> > suggestion was to place the
> > coverslip in xylene and re-attach it to the slide.
> > Our Director decided we
> > should return to glass coverslipping with an
> > appropriate mountant to try to
> > preserve the slides for the 20 years we are required
> > to keep them.
> >
> > Bill Sinai
> > Laboratory Manager
> > Tissue Pathology ICPMR
> > P.O. Box 533
> > Wentworthville NSW Australia 2145
> >
> >
> ############################################################################
> > ###############
> >
> > Subject: Re: Daily Digest
> >
> >
> > > We have had the Tissue Tek coverslipper now for
> > about 4 years. I have
> > > looked at the slide we first coverslipped with it
> > and have seen no
> > > problems. We did do some environmental changes to
> > make sure that the
> > > manufactures suggestions on storage were met
> > (temperature and humidity).
> > > I think this is very important.
> > >
> > > Rae Ann Staskiewicz HT(ASCP)
> > > Galesburg Animal Disease Lab
> > > Galesburg, IL
> > >
> > > Mrhabbs@aol.com wrote:
> > > >
> > > > My lab is looking at the Sakura Tissue Tek
> > automatic coverslipper
> > (tape).
> > > > Could I please get some feedback on the long
> > term quality of sections
> > and any
> > > > other comments that people who have experience
> > with
> === message truncated ===
>
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