RE: Daily Digest
| From: | "Viprino, Richard" <RickViprino@chi-east.org> |
I am NOT mad at you. OK?
AMY
-----Original Message-----
From: HistoNet Server [mailto:histonet@pathology.swmed.edu]
Sent: Tuesday, February 06, 2001 11:59 PM
To: HistoNet Server
Subject: Daily Digest
----------------------------------------------------------------------
Date: 6 Feb 2001 03:46:00 -0600
From: "Brennan, Liam" <Liam.Brennan@bll.n-i.nhs.uk>
Subject: Organ Retention
6/02/01
Histonetters-
Recently in the UK the national press has been focusing on the issue
of organ retention(particularly baby organs), after post-mortem examination,
by some hospital pathology departments without the informed consent of the
relatives. This has been sparked by the issue of a report into the
stock-piling of organs for no apparent reason by a pathologist at Alder Hey
Hospital in Liverpool, and the subsequent revealation by several other
hospital's that they retained organs without informed consent of the
relatives. This has resulted in hospitals being swamped with phone calls
from anxious relatives enquiring if the organs of any of their loved ones,
who underwent post-mortem examination (some as far back as 40- 50 years)
have been retained. Fortunately our pathology department does not retain
organs. However I would be interested to hear the views, policies and
procedures of the international community with regard to this issue.
Liam Brennan
Histopathology Dept.
Belfast City Hospital
----------------------------------------------------------------------
Date: 6 Feb 2001 05:00:30 -0600
From: "V#233#ronique" Wunderle <vwunderle@yahoo.com>
Subject: H&E staining
hi everybody !
I am pretty new in the "histology world" and I am
slowly discovering all the amazing techniques which
are out there.
I am starting with a basic one : H&E staining (for
histology and counterstaining).
I have been looking around for a protocol and as usual
there is not one protocol but multiple variations on
the theme !
can anyone tell me why some people, after hematoxylin
staining and rinsing in tap water, go straight in
eosin whereas others bother to destain and
differentiate in acid alcohol, wash in H2O, blue up in
lithium carbonate or ammonia water (which one is the
best ?)before going in eosin ?
Time of staining in hematoxylin also varies from one
proto to the next. Is it possible to overstain ?
thanks.
veronique.
=====
Veronique M. Wunderle, PhD
Responsable du reseau Nucleis
tel (0)2 41 35 56 82
fax (0)2 41 35 41 38
__________________________________________________
Do You Yahoo!?
Yahoo! Auctions - Buy the things you want at great prices.
http://auctions.yahoo.com/
----------------------------------------------------------------------
Date: 6 Feb 2001 06:17:40 -0600
From: "Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>
Subject: Fwd: recommendations for cryostats
Rita,
You have a lucky colleague. My 1974 Bright cryostat operates as
perfectly as the day it was installed. If I ever have to replace it, Bright
will be the instrument of first choice. Need I say more.
Ian.
>Date: Mon, 05 Feb 2001 18:37:25 -0400
>From: angelrj@email.uc.edu
>Subject: recommendations for cryostats
>To: histonet@pathology.swmed.edu
>
>Dear Histonetters,
>
>I have a colleague that is interested in purchasing a cryostat. I've
>only had experience with one brand and feel unable to be of much help.
>Can you please recommend a company or model that you've had experience
>with, or one you highly recommend? Thank you in advance!
>
>Rita Angel, HT
>University of Cincinnati
Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855. Extn:6602.
Fax: 0141 330 2923
e-mail: ian.montgomery@bio.gla.ac.uk
******************* NOTE *******************
There may be important message content
contained in the following MIME Information.
********************************************
- ------------------ MIME Information follows ------------------
- --=====================_7306989==_.ALT
Content-Type: text/plain; charset="us-ascii"; format=flowed
<<<<<< See above "Message Body" >>>>>>
- --=====================_7306989==_.ALT
Content-Type: text/html; charset="us-ascii"
<html>
Rita,<br>
<x-tab> </x-tab>You have a
lucky colleague. My 1974 Bright cryostat operates as perfectly as the day
it was installed. If I ever have to replace it, Bright will be the
instrument of first choice. Need I say more.<br>
Ian. <br>
<blockquote type=cite class=cite cite>Date: Mon, 05 Feb 2001 18:37:25
- -0400<br>
From: angelrj@email.uc.edu<br>
Subject: recommendations for cryostats<br>
To: histonet@pathology.swmed.edu<br>
<br>
Dear Histonetters,<br>
<br>
I have a colleague that is interested in purchasing a cryostat.
I've<br>
only had experience with one brand and feel unable to be of much help.
<br>
Can you please recommend a company or model that you've had
experience<br>
with, or one you highly recommend? Thank you in advance!<br>
<br>
Rita Angel, HT<br>
University of Cincinnati</blockquote>
<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
West Medical Building,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 339 8855. Extn:6602.<br>
Fax: 0141 330 2923<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>
- --=====================_7306989==_.ALT--
----------------------------------------------------------------------
Date: 6 Feb 2001 07:45:32 -0600
From: "Teri Johnson" <terij@prlnet.com>
Subject: Re: headphones in Histology
I agree with this view as well. The problem with having the radio on is it
seems everybody likes a different radio station and it's difficult to find
something everyone can agree on. As long as everybody understands the
"rules" I don't see this to be much of a problem.
- -Teri Johnson
Physicians Reference Laboratory
Overland Park, KS
- ----- Original Message -----
From: <Barbara.Davies@memhospcs.org>
To: <histonet@pathology.swmed.edu>
Sent: Monday, February 05, 2001 2:31 PM
Subject: re: headphones in Histology
> Headphones are allowed in our very busy histology lab. One person
> frequently takes advantage of this and it has never been a problem. He
> has always kept up with the conversation at hand and has always responded
> to the frozens, kidneys, muscle or pathologist stat needs without delay or
> any problem. We would change the ruling if a serious problem presented
> itself...but so far, so good. (We have been doing this for years!) This
> individual works better with constant noise as opposed to some of the
rest
> of us who work better with quiet. To each his own!
>
> Barb Davies
> Memorial Hospital
> Colorado Springs, CO
>
>
>
----------------------------------------------------------------------
Date: 6 Feb 2001 09:00:31 -0600
From: "Rippstein, Peter" <prippstein@ottawahospital.on.ca>
Subject: Tissue Processor Alarms
Histoneters,
I would like to get a general consensus on the merits of external alarms on
tissue processors which would alert staff in case of an error during the
night or on weekends.
Thanking you in advance,
Peter Rippstein, ART
Charge Technologist
Anatomical Pathology
The Ottawa Hospital, Civic Campus
Ph: 798-5555 ext 16589
Fax: 761-4846
email: prippstein@ottawahospital.on.ca
----------------------------------------------------------------------
Date: 6 Feb 2001 09:18:23 -0600
From: "Barry Rittman" <brittman@mail.db.uth.tmc.edu>
Subject: Re: Xylene usage
I come from a generation that usually erred on the side of caution when
using
xylene.
I believe that whatever works for you is what you should go with.
I do have two concerns:
I do not work in a certified lab (certifiable people certainly). Is there a
requirement re the volume of xylene that can be used for x number of blocks?
Most of the discussions revolve around tissues that are cut and stained
within
a
short time. As most of the processing in path labs is somewhat rapid there
is
a
likelihood that traces of xylene will be left in the tissue. If this xylene
has
numerous contaminants will this not affect the future use of the blocks?
I have no direct knowledge of this but put this up for discussion.
As far as using xylene for clearing sections, I am a firm believer in using
as
fresh xylene as possible.
Thanks
Barry
----------------------------------------------------------------------
Date: 6 Feb 2001 09:18:40 -0600
From: Geoff McAuliffe <mcauliff@UMDNJ.EDU>
Subject: Re: H&E staining
V#233#ronique Wunderle wrote:
> hi everybody !
> I am pretty new in the "histology world" and I am
> slowly discovering all the amazing techniques which
> are out there.
> I am starting with a basic one : H&E staining (for
> histology and counterstaining).
> I have been looking around for a protocol and as usual
> there is not one protocol but multiple variations on
> the theme !
> can anyone tell me why some people, after hematoxylin
> staining and rinsing in tap water, go straight in
> eosin whereas others bother to destain and
> differentiate in acid alcohol, wash in H2O, blue up in
> lithium carbonate or ammonia water (which one is the
> best ?)before going in eosin ?
> Time of staining in hematoxylin also varies from one
> proto to the next. Is it possible to overstain ?
> thanks.
> veronique.
>
Hi Veronique:
To my knowledge there are two types of hematoxylin staining.
Progressive staining is done with an "acid iron chloride" type of
Hematoxylin like Weigerts. The stain is self-limiting and does not need
to be destained. The second type is Regressive staining, done with an
alum type of hematoxylin like Delafield's. After staining, the nuclear
stain is differentiated with very dilute acid to remove excess stain
from unwanted structures, then "blued" in a mild alkali (Scott's
solution for example). There are many, many variations on each type of
hematoxylin. Find one that gives results you like and stick with it.
I also suggest an introductory textbook for your edification.
Humason's "Animal Tissue Techniques" is excellent. Kiernan's
"Histological and Histochemical Methods" is also excellent, but much
more advanced.
Geoff
- --
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************
----------------------------------------------------------------------
Date: 6 Feb 2001 09:19:01 -0600
From: cmcbride@tulane.edu
Subject: ISH using a PCR machine
Hi!
Does anyone have a protocol using a PCR machine for section ISH?
Thanks,
Christine
Christine McBride, M.S.
Tulane University Medical Center
Center for Gene Therapy
SL-99
1430 Tulane Ave.
New Orleans, LA 70112
cmcbride@tulane.edu
Phone:(504) 988-7069
Fax:(504) 588-5326
----------------------------------------------------------------------
Date: 6 Feb 2001 10:12:53 -0600
From: cmcbride@tulane.edu
Subject: Extracting DNA from bone
Hi,
Does anyone know how to grind bone into a fine enough powder to extract DNA
from it?
Thanks,
Christine
Christine McBride, M.S.
Tulane University Medical Center
Center for Gene Therapy
SL-99
1430 Tulane Ave.
New Orleans, LA 70112
cmcbride@tulane.edu
Phone:(504) 988-7069
Fax:(504) 588-5326
----------------------------------------------------------------------
Date: 6 Feb 2001 10:13:09 -0600
From: "Vicki Gauch" <GauchV@mail.amc.edu>
Subject: Re: Tissue Processor Alarms
Peter,
We have external alarms on all of our processors as well as our -70
freezer.
We have a tech on call at all times and those alarms have saved us more
times
than I care to mention. Since we are notified of any fault in the
processors,
we can then come in, assess the situation and move the tissues to another
processor if need be...thus saving our TAT on those cases which would
otherwise have to be processed the following day or on Monday in the case of
a
weekend problem. It has also allowed us to have the processors looked at on
off hours so the repair process could be initiated rather than having to
wait
until the following work day. I am very much in favor of the
alarms.....having worked both with and without them....they can make all the
difference in the world...
Have a great day,
Vicki
Albany Medical Center
>>> "Rippstein, Peter" <prippstein@ottawahospital.on.ca> 02/06/01 09:52AM
>>>
Histoneters,
I would like to get a general consensus on the merits of external alarms on
tissue processors which would alert staff in case of an error during the
night or on weekends.
Thanking you in advance,
Peter Rippstein, ART
Charge Technologist
Anatomical Pathology
The Ottawa Hospital, Civic Campus
Ph: 798-5555 ext 16589
Fax: 761-4846
email: prippstein@ottawahospital.on.ca
----------------------------------------------------------------------
Date: 6 Feb 2001 10:13:26 -0600
From: "Tricia Hughey" <tlhughey@home.com>
Subject: Grossing by non-pathologists.
Group: I would appreciate your help in determining the
qualifications/certifications required for grossing by non-pathologists.
Outside of the advanced Pathology Assistant's degree, under what conditions
can a non-physician perform grossing (under the technical supervision of a
pathologist) of course. Can a HT gross specimens, especially those that are
fully embedded? Can a non-HT do any grossing? Where can I find clear and
definitive guidelines? In our situation, this pertains almost entirely to
non-hospital specimens. Does this make a difference? Thank you for any
input. Tricia Hughey, Pathology Partners, Denver, CO
----------------------------------------------------------------------
Date: 6 Feb 2001 10:13:47 -0600
From: "Carson, Karla" <KCarson@chw.edu>
Subject: RE: Tissue Processor Alarms
We have them and they are very helpful if you are a big lab. They allow
someone to be called at home and get the processor back on line if possible
or switch to a back up unit. We are a regional lab and service 8 pathology
departments and not one of them is too understanding if there is a delay.
Karla Carson
Regional Pathology Manager
Mercy Health Care Sacramento
Phone 916-453-4494
FAX 916-453-4397
e-mail kcarson@chw.edu <mailto:kcarson@chw.edu>
-----Original Message-----
From: Rippstein, Peter
[mailto:prippstein@ottawahospital.on.ca]
Sent: Tuesday, February 06, 2001 6:53 AM
To: 'Histonet@pathology.swmed.edu'
Subject: Tissue Processor Alarms
Histoneters,
I would like to get a general consensus on the merits of
external alarms on
tissue processors which would alert staff in case of an
error during the
night or on weekends.
Thanking you in advance,
Peter Rippstein, ART
Charge Technologist
Anatomical Pathology
The Ottawa Hospital, Civic Campus
Ph: 798-5555 ext 16589
Fax: 761-4846
email: prippstein@ottawahospital.on.ca
----------------------------------------------------------------------
Date: 6 Feb 2001 10:14:03 -0600
From: Louise Hecker <lhecker@binghamton.edu>
Subject: plant histology
Hello,
I am a histologist at Binghamton University. I have a student that is
interested in doing some basic histology on soft plant leaves. She would
eventually like to do some histochemistry. My experience in histology has
only been with animals. Does anyone have a very general protocol for
plants? Such as: what fixatives to use, how to process the tissue, how to
infiltrate properly, etc. Any information would be useful. thanks,
Louise Hecker
Louise Hecker
Histology/Cell Biology/Bldg. Admin.
Department of Biological Sciences
Binghamton University
Binghamton, NY 13902-6000
(607)777-6207
Lhecker@binghamton.edu
----------------------------------------------------------------------
Date: 6 Feb 2001 10:14:21 -0600
From: "Jennifer Englin" <JLE@rice.willmar.mn.us>
Subject: Pitter-Patter...RE: HEADPHONES IN HISTOLOGY
Unprofessional and difficult to hear all the timers and beeps happening in
the
lab.
Jennifer Englin
----------------------------------------------------------------------
Date: 6 Feb 2001 10:14:38 -0600
From: "Jennifer MacDonald" <jmacdona@mtsac.edu>
Subject: Re: Tissue Processor Alarms
We have been saved more times than I care to mention by the external alarm.
There is no coverage in the histo lab at night so we had the alarm sound (by
remote) in the clinical lab. I receive a call at home by the lab staff.
Most of the times I can talk them through any problems. On occasion I did
have to go into the lab to fix the problem, but I would rather do that than
find the "problem" Monday morning.
Jennifer MacDonald
> I would like to get a general consensus on the merits of external alarms
on
> tissue processors which would alert staff in case of an error during the
> night or on weekends.
> Thanking you in advance,
>
> Peter Rippstein, ART
> Charge Technologist
> Anatomical Pathology
> The Ottawa Hospital, Civic Campus
> Ph: 798-5555 ext 16589
> Fax: 761-4846
> email: prippstein@ottawahospital.on.ca
>
>
----------------------------------------------------------------------
Date: 6 Feb 2001 12:14:43 -0600
From: Gayle Callis <uvsgc@montana.edu>
Subject: Re: headphones in Histology
This message hits home especially in my lab today after a move to another
area with more people in lab. Musical preferences vary greatly, and can
be very annoying/irritating to those who dislike a particular station. If
radio music is a source of irritation, this could be detrimental to work
production eventually since you can't escape a radio playing. People need
non stressful work conditions.
One laboratory supervisor, some years ago, had this problem, people
fighting over what radio station to listen to, cytotechs sitting a
microscopes were working in an area close to histo group. He permitted
people to work with headphones/their musical choices. Peace was restored
along with work productivity.
1. Headphones can be used so normal conversation/other sounds get
through.
Been there, done that!
2. Agree with comments about hearing impaired, are they a safety risk?
3. Plugging into my headphones today to escape a radio constantly
playing
the same tunes over and over - I need to ESCAPE this onslaught.
One observation about headphone users is a reluctance by others who want to
ask them something. The askers) tend to backoff, as headphones can
indicate do not interrupt/privacy boundaries. Headphone users have to be
aware this is not the case IF IT IS UNDERSTOOD and VOLUME IS LOW, they have
to respond to people wanting to talk to them.
If headphones are ultimately not allowed, then radios should be banned
also. I would rather have total silence than music that wears me down, ad
nauseum.
My 25 cents worth, off to my CD/radio player.
At 07:23 AM 2/6/01 -0600, you wrote:
>I agree with this view as well. The problem with having the radio on is it
>seems everybody likes a different radio station and it's difficult to find
>something everyone can agree on. As long as everybody understands the
>"rules" I don't see this to be much of a problem.
>
>-Teri Johnson
>Physicians Reference Laboratory
>Overland Park, KS
>
Gayle Callis
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610
406 994-6367
404 994-4303 (FAX)
----------------------------------------------------------------------
Date: 6 Feb 2001 12:15:04 -0600
From: "Morken, Tim" <tim9@cdc.gov>
Subject: RE: Tissue Processor Alarms
I would add to this that having histology people do the on-call is
best,IMHO. I worked in a hospital where the night shift clinical lab people
would check our processor if the alarm went off but it did not work out very
well - they just did not have the vested interest to do a good job of it.
Histology people will be motivated to take care of the problem rather than
ignore it.
Tim
- -----Original Message-----
From: Vicki Gauch [mailto:GauchV@mail.amc.edu]
Sent: Tuesday, February 06, 2001 10:10 AM
To: prippstein@ottawahospital.on.ca; histonet@pathology.swmed.edu
Subject: Re: Tissue Processor Alarms
Peter,
We have external alarms on all of our processors as well as our -70
freezer. We have a tech on call at all times and those alarms have saved us
more times than I care to mention. Since we are notified of any fault in
the processors, we can then come in, assess the situation and move the
tissues to another processor if need be...thus saving our TAT on those cases
which would otherwise have to be processed the following day or on Monday in
the case of a weekend problem. It has also allowed us to have the
processors looked at on off hours so the repair process could be initiated
rather than having to wait until the following work day. I am very much in
favor of the alarms.....having worked both with and without them....they can
make all the difference in the world...
Have a great day,
Vicki
Albany Medical Center
>>> "Rippstein, Peter" <prippstein@ottawahospital.on.ca> 02/06/01 09:52AM
>>>
Histoneters,
I would like to get a general consensus on the merits of external alarms on
tissue processors which would alert staff in case of an error during the
night or on weekends.
Thanking you in advance,
Peter Rippstein, ART
Charge Technologist
Anatomical Pathology
The Ottawa Hospital, Civic Campus
Ph: 798-5555 ext 16589
Fax: 761-4846
email: prippstein@ottawahospital.on.ca
----------------------------------------------------------------------
Date: 6 Feb 2001 12:15:26 -0600
From: Roger Moretz <stamptrain@yahoo.com>
Subject: Re: Tissue Processor Alarms
Rather than re-write it, I'll just second Vicki's
comments.
Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals
- --- Vicki Gauch <GauchV@mail.amc.edu> wrote:
> Peter,
> We have external alarms on all of our processors as
> well as our -70 freezer. We have a tech on call at
> all times and those alarms have saved us more times
> than I care to mention. Since we are notified of
> any fault in the processors, we can then come in,
> assess the situation and move the tissues to another
> processor if need be...thus saving our TAT on those
> cases which would otherwise have to be processed the
> following day or on Monday in the case of a weekend
> problem. It has also allowed us to have the
> processors looked at on off hours so the repair
> process could be initiated rather than having to
> wait until the following work day. I am very much
> in favor of the alarms.....having worked both with
> and without them....they can make all the difference
> in the world...
>
> Have a great day,
> Vicki
> Albany Medical Center
>
> >>> "Rippstein, Peter"
> <prippstein@ottawahospital.on.ca> 02/06/01 09:52AM
> >>>
> Histoneters,
>
> I would like to get a general consensus on the
> merits of external alarms on
> tissue processors which would alert staff in case of
> an error during the
> night or on weekends.
> Thanking you in advance,
>
> Peter Rippstein, ART
> Charge Technologist
> Anatomical Pathology
> The Ottawa Hospital, Civic Campus
> Ph: 798-5555 ext 16589
> Fax: 761-4846
> email: prippstein@ottawahospital.on.ca
>
>
>
>
__________________________________________________
Do You Yahoo!?
Yahoo! Auctions - Buy the things you want at great prices.
http://auctions.yahoo.com/
----------------------------------------------------------------------
Date: 6 Feb 2001 12:15:48 -0600
From: Geoff McAuliffe <mcauliff@UMDNJ.EDU>
Subject: Re: plant histology
Louise Hecker wrote:
> Hello,
> I am a histologist at Binghamton University. I have a student that is
> interested in doing some basic histology on soft plant leaves. She would
> eventually like to do some histochemistry. My experience in histology has
> only been with animals. Does anyone have a very general protocol for
> plants? Such as: what fixatives to use, how to process the tissue, how to
> infiltrate properly, etc. Any information would be useful. thanks,
> Louise Hecker
>
> Louise Hecker
> Histology/Cell Biology/Bldg. Admin.
> Department of Biological Sciences
> Binghamton University
> Binghamton, NY 13902-6000
> (607)777-6207
> Lhecker@binghamton.edu
"Botanical Microtechnique and Cytochemistry" by Graeme P. Berlyn is pretty
much the classic in the field. The latest edition is 1976 (?) but I think it
is still in print.
Geoff
- --
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************
----------------------------------------------------------------------
Date: 6 Feb 2001 13:26:19 -0600
From: "Terrett, Barb" <Barb.Terrett@uhn.on.ca>
Subject: RE: headphones in Histology
We actually had a hearing impaired tech wearing headphones to listen to
music while working. Needless to say, this did not work out as she was
expected to answer the phone, respond to coworkers etc. just like everyone
else. The supervisors decided it was inappropriate for her to use headphones
in the lab, and to be fair, the rule was applied to all. If the radio is to
be on, all have to agree to the station or it is turned off.
> -----Original Message-----
> From: Gayle Callis [SMTP:uvsgc@montana.edu]
> Sent: February 6, 2001 11:22 AM
> To: terij@prlnet.com; histonet@pathology.swmed.edu
> Subject: Re: headphones in Histology
>
> This message hits home especially in my lab today after a move to another
> area with more people in lab. Musical preferences vary greatly, and can
> be very annoying/irritating to those who dislike a particular station. If
> radio music is a source of irritation, this could be detrimental to work
> production eventually since you can't escape a radio playing. People need
> non stressful work conditions.
>
> One laboratory supervisor, some years ago, had this problem, people
> fighting over what radio station to listen to, cytotechs sitting a
> microscopes were working in an area close to histo group. He permitted
> people to work with headphones/their musical choices. Peace was restored
> along with work productivity.
>
> 1. Headphones can be used so normal conversation/other sounds get
> through.
> Been there, done that!
>
> 2. Agree with comments about hearing impaired, are they a safety risk?
>
>
> 3. Plugging into my headphones today to escape a radio constantly
> playing
> the same tunes over and over - I need to ESCAPE this onslaught.
>
> One observation about headphone users is a reluctance by others who want
> to
> ask them something. The askers) tend to backoff, as headphones can
> indicate do not interrupt/privacy boundaries. Headphone users have to be
> aware this is not the case IF IT IS UNDERSTOOD and VOLUME IS LOW, they
> have
> to respond to people wanting to talk to them.
>
> If headphones are ultimately not allowed, then radios should be banned
> also. I would rather have total silence than music that wears me down, ad
> nauseum.
>
> My 25 cents worth, off to my CD/radio player.
>
>
>
> At 07:23 AM 2/6/01 -0600, you wrote:
> >I agree with this view as well. The problem with having the radio on is
> it
> >seems everybody likes a different radio station and it's difficult to
> find
> >something everyone can agree on. As long as everybody understands the
> >"rules" I don't see this to be much of a problem.
> >
> >-Teri Johnson
> >Physicians Reference Laboratory
> >Overland Park, KS
> >
>
> Gayle Callis
> Veterinary Molecular Biology
> Montana State University - Bozeman
> Bozeman MT 59717-3610
>
> 406 994-6367
> 404 994-4303 (FAX)
>
----------------------------------------------------------------------
Date: 6 Feb 2001 13:26:37 -0600
From: "LaFriniere, Mike" <Mike_LaFriniere@memorial.org>
Subject: RE: Grossing by non-pathologists.
Tracy,
CLIA rulings and CAP guidelines may be best to find the answers you are
looking for. If I remember correctly, if you were an HT(ASCP) performing
grossing duties prior to CLIA ruling, you were "grandfathered" in, such with
regards to the types of specimens one may gross. For example if one was
performing all grossing duties of all organ systems prior to the CLIA ruling
on "non pathologists performing gross prosecting" you may continue to do so
as long as you have such proof of experience and it is under the direct
supervision of a pathologist. However CLIA has implemented a date I think in
the mid 1990's that after this date and not grandfathered in, you must have
a science degree to perform such "advanced" testing.
Now, the question is what is advanced testing? This is a good question, I
would take advance testing or (grossing) to be considered as whole organs
that demonstrate major neoplastic disease (kidneys, lungs, colons etc). As a
Manager, CAP inspector, and a gross prosector myself, Our policy allows most
of all small specimens, skins, GI bx's, gyn bx's, bones and up to routine
gallballders to include non carcinoma hysterectomies, and colons with
diverticula be considered "non-complicated" specimens that can be grossed by
a HT(ASCP)under the direct supervision of a pathologist who has been
grandfathered in and demonstrates the appropriate experience. However this
area is very subjected and the person performing prosecting and should have
appropriate documentation of training by a pathologist to include a yearly
evaluation. Additional information is noted on page 6 of the 38 page CAP
check off list that require a CAP lab to have documentation of which type of
specimens are examined by a "non pathologist" I will be happy to fax this to
you if you do not have a copy.
Michael R. LaFriniere
Manager Dept Pathology
Memorial Hospital, Chattanooga
- -----Original Message-----
From: Tricia Hughey [mailto:tlhughey@home.com]
Sent: Tuesday, February 06, 2001 10:14 AM
To: histonet@pathology.swmed.edu
Subject: Grossing by non-pathologists.
Group: I would appreciate your help in determining the
qualifications/certifications required for grossing by non-pathologists.
Outside of the advanced Pathology Assistant's degree, under what conditions
can a non-physician perform grossing (under the technical supervision of a
pathologist) of course. Can a HT gross specimens, especially those that are
fully embedded? Can a non-HT do any grossing? Where can I find clear and
definitive guidelines? In our situation, this pertains almost entirely to
non-hospital specimens. Does this make a difference? Thank you for any
input. Tricia Hughey, Pathology Partners, Denver, CO
----------------------------------------------------------------------
Date: 6 Feb 2001 13:27:03 -0600
From: "Ryan.Linda" <ryan2@niehs.nih.gov>
Subject: RE: headphones in Histology
I found this topic extremely interesting. The issue of headphones
being raised as a safety factor. It does not pose a safety danger. I
work with in a lab with a severe hearing impaired technologist. She
has been in the field for over 20 years. There has never been a
safety incident due to her impairment. It boils down to social
interactions between co-workers. The headphones will likely decrease
verbal dialogs between coworkers but to imply a safety factor is
simply not the case.
> ----------
> From: Gayle Callis
> Sent: Tuesday, February 6, 2001 11:22 AM
> To: terij@prlnet.com; histonet@pathology.swmed.edu
> Subject: Re: headphones in Histology
>
> This message hits home especially in my lab today after a move to
> another
> area with more people in lab. Musical preferences vary greatly,
> and can
> be very annoying/irritating to those who dislike a particular
> station. If
> radio music is a source of irritation, this could be detrimental to
> work
> production eventually since you can't escape a radio playing.
> People need
> non stressful work conditions.
>
> One laboratory supervisor, some years ago, had this problem, people
> fighting over what radio station to listen to, cytotechs sitting a
> microscopes were working in an area close to histo group. He
> permitted
> people to work with headphones/their musical choices. Peace was
> restored
> along with work productivity.
>
> 1. Headphones can be used so normal conversation/other sounds get
> through.
> Been there, done that!
>
> 3. Plugging into my headphones today to escape a radio constantly
> playing
> the same tunes over and over - I need to ESCAPE this onslaught.
>
> One observation about headphone users is a reluctance by others who
> want to
> ask them something. The askers) tend to backoff, as headphones can
> indicate do not interrupt/privacy boundaries. Headphone users have
> to be
> aware this is not the case IF IT IS UNDERSTOOD and VOLUME IS LOW,
> they have
> to respond to people wanting to talk to them.
>
> If headphones are ultimately not allowed, then radios should be
> banned
> also. I would rather have total silence than music that wears me
> down, ad
> nauseum.
>
> My 25 cents worth, off to my CD/radio player.
>
>
>
> At 07:23 AM 2/6/01 -0600, you wrote:
> >I agree with this view as well. The problem with having the radio
> on is it
> >seems everybody likes a different radio station and it's difficult
> to find
> >something everyone can agree on. As long as everybody understands
> the
> >"rules" I don't see this to be much of a problem.
> >
> >-Teri Johnson
> >Physicians Reference Laboratory
> >Overland Park, KS
> >
>
> Gayle Callis
> Veterinary Molecular Biology
> Montana State University - Bozeman
> Bozeman MT 59717-3610
>
> 406 994-6367
> 404 994-4303 (FAX)
>
>
>
----------------------------------------------------------------------
Date: 6 Feb 2001 14:26:44 -0600
From: "Brody,Juanita X" <Juanita.X.Brody@kp.org>
Subject: BK Polyoma Antibody
Does anyone know a source or reference lab(west coast) that has this
antibody.
Thanks in Advance
----------------------------------------------------------------------
Date: 6 Feb 2001 14:27:02 -0600
From: "Su, Phy-Huynh" <psu@shctampa.usf.edu>
Subject: RE: Extracting DNA from bone
We mince fresh bone or cartilage tissues in PBS finely first, with razor
blades held by a hemostat, or by #20 scalpel. Remove the PBS as much as
possible, pool the minced tissues in a 50ml POLYPROPYLENE tube (weigh them
if you need to at this point), and freeze the tissues in tube in liquid
nitrogen (polysterene tube will break).
Next, we pool a little bit of tissues (sometimes it's very hard to scrap
tissues out from the tubes, so we smash and break the bottom of the tube
with a hammer) into a chilled, metal puvelrizer (?) in a dry ice box. Then
using a hammer, we smash the tissues manually (requires a lot of muscle) to
powder, and remove the powder with a chilled spatula into chilled tubes. At
this point, you can keep the tissues frozen (-80 degree C) if running late.
Another way (better, but need instrument): using a magnetic pulverizer in a
liquid nitrogen bath. From the wet, weighed tissues, we can put tissues in
pulverizing tubes, immerse into liquid nitrogen, and pulverize. This is
better because the powder is much finer, requires less strength, and faster.
But you need the instrument.
Finally, we use a tissue homogenizer to homogenize tissues in extracting
buffer, either for DNA, RNA, or protein.
If you need details, tricks here and there, give me a call. I'll be happy
to help.
Su
> -----Original Message-----
> From: cmcbride@tulane.edu [SMTP:cmcbride@tulane.edu]
> Sent: Tuesday, February 06, 2001 10:13 AM
> To: histonet
> Subject: Extracting DNA from bone
>
> Hi,
> Does anyone know how to grind bone into a fine enough powder to extract
> DNA
> from it?
> Thanks,
> Christine
>
> Christine McBride, M.S.
> Tulane University Medical Center
> Center for Gene Therapy
> SL-99
> 1430 Tulane Ave.
> New Orleans, LA 70112
> cmcbride@tulane.edu
> Phone:(504) 988-7069
> Fax:(504) 588-5326
----------------------------------------------------------------------
Date: 6 Feb 2001 14:27:18 -0600
From: NLOUISEA@aol.com
Subject: Shurmount
Hello
We are unable to get Shurmount from our current supplier. Does anyone know
of a source that has Shurmount in stock? and/or what other mounting media
works as well on a Hacker coverslipper?
Thanks
Nancy
----------------------------------------------------------------------
Date: 6 Feb 2001 14:27:35 -0600
From: NLOUISEA@aol.com
Subject: xylene distilling
Hello
We are demo-ing a CBG Biotech xylene distiller. Any comments from anybody
using one? or did anyone chose another brand? pros? cons?
Thanks
Nancy
----------------------------------------------------------------------
Date: 6 Feb 2001 15:41:43 -0600
From: Patti Loykasek <ploykasek@phenopath.com>
Subject: Re: BK Polyoma Antibody
on 2/6/01 11:20 AM, Brody,Juanita X at Juanita.X.Brody@kp.org wrote:
> Does anyone know a source or reference lab(west coast) that has this
> antibody.
> Thanks in Advance
>
We use a polyclonal antibody from Access Biomedical- San Diego. It works for
us at 1:1000 using no pretreatment.
Patti Loykasek
Phenopath Labs
----------------------------------------------------------------------
Date: 6 Feb 2001 15:42:14 -0600
From: "Bennett, Catherine (Katie)" <cbennett@lrri.org>
Subject: Cromogen/Substrate IHC question
Advice needed:
If I switch an IHC procedure over from an ABC-HRP kit to an ABC-Alk Phos
kit, I shouldn't have to adjust my primary and secondary antibody
concentrations, right? (I want to change cromogen labels from DAB to
Vector-Red.)
TIA.
*********************************
Catherine "Katie" Bresee Bennett
Sr. Research Technologist
Lovelace Respiratory Research Institute
Albuquerque, New Mexico
----------------------------------------------------------------------
Date: 6 Feb 2001 16:27:06 -0600
From: "Michael Rice" <MRICE@nbhd.org>
Subject: Re: Cryostat information
We just received our Leica cryostat, eay to use and coftorable to work with,
even pathologists can get great sections now
Mike
>>> Steven Postl <steven.p.postl@abbott.com> 02/01/01 02:35PM >>>
Our laboratory is looking to purchase a new cryostat for research
applications. Can you cryostat guru's share your pro's and con's to help
guide us in our selection? Once again, Vendors, don't hesitate to drop a
line. We are looking to purchase within the first quarter. Many thanks!
Steve Postl and Amy Camarato
Abbott Laboratories
1-847-937-3888
******************* NOTE *******************
There may be important message content
contained in the following MIME Information.
********************************************
- ------------------ MIME Information follows ------------------
- --=_3A618D99.1F7E1D83
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: quoted-printable
<<<<<< See above "Message Body" >>>>>>
- --=_3A618D99.1F7E1D83
Content-Type: text/html
Content-Disposition: attachment; filename="TEXT.htm"
Content-Description: HTML
<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
<HTML><HEAD>
<META content="text/html; charset=iso-8859-1" http-equiv=Content-Type>
<META content="MSHTML 5.00.2919.6307" name=GENERATOR></HEAD>
<BODY style="FONT: 8pt MS Sans Serif; MARGIN-LEFT: 2px; MARGIN-TOP: 2px">
<DIV><FONT size=1>We just received our Leica cryostat, eay to use and
coftorable
to work with, even pathologists can get great sections now</FONT></DIV>
<DIV><FONT size=1>Mike</FONT><BR><BR>>>> Steven Postl
<steven.p.postl@abbott.com> 02/01/01 02:35PM >>><BR>Our
laboratory is looking to purchase a new cryostat for
research<BR>applications. Can you cryostat guru's share your pro's and
con's to help<BR>guide us in our selection? Once again, Vendors, don't
hesitate to drop a<BR>line. We are looking to purchase within the
first
quarter. Many thanks!<BR><BR>Steve Postl and Amy Camarato<BR>Abbott
Laboratories<BR>1-847-937-3888<BR><BR><BR></DIV></BODY></HTML>
- --=_3A618D99.1F7E1D83--
----------------------------------------------------------------------
Date: 6 Feb 2001 16:27:23 -0600
From: Jill Songer <jtsonger@vt.edu>
Subject: Re: Shurmount
We use the Richard Allan Mounting Media on the Hacker coverslipper.
At 02:30 PM 2/6/2001 -0500, NLOUISEA@aol.com wrote:
>Hello
>We are unable to get Shurmount from our current supplier. Does anyone know
>of a source that has Shurmount in stock? and/or what other mounting media
>works as well on a Hacker coverslipper?
>Thanks
>Nancy
**************************************************************************
Jill Songer HT (ASCP)
Virginia Tech
Veterinary Teaching Hospital
Anatomic Pathology Lab Supervisor
Blacksburg, Virginia 24061
GO HOKIES!!!!!!
----------------------------------------------------------------------
Date: 6 Feb 2001 16:27:43 -0600
From: "Ford, Judi {Path~Palo Alto}" <JUDI.FORD@ROCHE.COM>
Subject: RE: xylene distilling
We have a CBG distiller for xylene, alcohol and Clear-Rite lll. Aside from
using tech time we like using it. We've cut way back on solvent waste along
with cutting way down on purchasing solvents. We find we have to clean out
the filter quite frequently. The machine is quiet. The smell isn't
objectionable. Ergonomically its hard lifting containers up and down (we
lowered the counter). The CBG people are very helpful and concerned when we
have problems. The only problem we've really was overheating but we've
remedied that by replacing the fan.
Hope this helps.
Judi Ford
Palo Alto, CA
- -----Original Message-----
From: NLOUISEA@aol.com [mailto:NLOUISEA@aol.com]
Sent: Tuesday, February 06, 2001 11:35 AM
To: histonet@pathology.swmed.edu
Subject: xylene distilling
Hello
We are demo-ing a CBG Biotech xylene distiller. Any comments from anybody
using one? or did anyone chose another brand? pros? cons?
Thanks
Nancy
----------------------------------------------------------------------
Date: 6 Feb 2001 16:28:00 -0600
From: Charles.Embrey@carle.com
Subject: RE:xylene distilling
In my opinion the CBG unit is the absolute best money can buy. The quality
is
second to none. Customer service has always been a great. I have ordered 2
units in the past and am ordering one at my new facility. Stay away from
B&R.
Charles R. Embrey, Jr., PA(AAPA), HT(ASCP)
Carle Clinic
Urbana, IL
----------------------------------------------------------------------
Date: 6 Feb 2001 16:28:19 -0600
From: Joan Yonchek <Jyonchek@rtitechnology.com>
Subject: MHC-I
Does anyone have a source for MHC-I or MHC-II that works on FFPP tissue?
Thanks
Joan
RTI
----------------------------------------------------------------------
Date: 6 Feb 2001 16:28:36 -0600
From: Tony Henwood <AnthonyH@chw.edu.au>
Subject: Tissue Processor Alarms -Reply
Petr,
We have our processors as well as our -70oC Freezer on an external
alarm, attached to a pager that staff on call keep with them.
Being a Childrens Hospital, we often have smaller biopsies to process as
well as little chance of having them recollected. There is also a time
factor where a delay of 6-8hrs would cause more distress for a child
then for an adult.
We can't really afford delay or a possibility of losing these biopsies. The
Pager helps to maintain at least some peace of mind.
Tony
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital Westmead,
Locked Bag 4001, Westmead, Australia, 2145
Tel: (02) 9845 3306
Fax: (02) 9845 3318
>>> "Rippstein, Peter" <prippstein@ottawahospital.on.ca>
7/February/2001 12:52am >>>
Histoneters,
I would like to get a general consensus on the merits of external alarms
on
tissue processors which would alert staff in case of an error during the
night or on weekends.
Thanking you in advance,
Peter Rippstein, ART
Charge Technologist
Anatomical Pathology
The Ottawa Hospital, Civic Campus
Ph: 798-5555 ext 16589
Fax: 761-4846
email: prippstein@ottawahospital.on.ca
----------------------------------------------------------------------
Date: 6 Feb 2001 16:29:06 -0600
From: Amos & Theresa <atbrooks@snet.net>
Subject: Xylene usage
Hi,
Regarding xylene usage, one should always remember that a chemical
becomes diluted with the previous chemical after every use. There is a
carryover of about 100 ml (in many cases more, depending on the
processor and how many tissues are in the retort) of solution from one
step to the next. Most staining and sectioning problems can be linked to
this. I do agree that one should be as conservative as possible with the
hazardous chemicals. That is the ecological and economical responsible
thing to do. However, it must be stressed that in many cases a patient's
very life is in that retort, so you don't want to gamble with such a
simple thing to fix.
As a rule I always start a processor with fresh reagents in the last
group of chemicals. Rotate the rest as you want, but the last chemical,
at least, should be as fresh as possible.
Amos Brooks
----------------------------------------------------------------------
Date: 6 Feb 2001 16:29:23 -0600
From: Amos & Theresa <atbrooks@snet.net>
Subject: Re: Organ Retention
Hi,
My opinion for what it is worth... Let the dead (or what is left of them
post autopsy) be buried and be done with. There is no real identity to a
piece
of tissue in a formalin (or whatever chemical) container. Once the
individual
is
buried the deal is done. Anything left behind for educational purposes will
be
needed neither by the dead nor the living relatives, so why should someone
have
a problem with it.
The exception to this case would be for any cloning. I think there is a
reasonable common sense limitation there. I am not necessarily opposed to
cloning humans, I just dont think it should be done without consent.
Amos Brooks
"Brennan, Liam" wrote:
> 6/02/01
>
> Histonetters-
> Recently in the UK the national press has been focusing on the
issue
> of organ retention(particularly baby organs), after post-mortem
examination,
> by some hospital pathology departments without the informed consent of the
> relatives. This has been sparked by the issue of a report into the
> stock-piling of organs for no apparent reason by a pathologist at Alder
Hey
> Hospital in Liverpool, and the subsequent revealation by several other
> hospital's that they retained organs without informed consent of the
> relatives. This has resulted in hospitals being swamped with phone calls
> from anxious relatives enquiring if the organs of any of their loved ones,
> who underwent post-mortem examination (some as far back as 40- 50 years)
> have been retained. Fortunately our pathology department does not retain
> organs. However I would be interested to hear the views, policies and
> procedures of the international community with regard to this issue.
>
> Liam Brennan
> Histopathology Dept.
> Belfast City Hospital
----------------------------------------------------------------------
Date: 6 Feb 2001 18:29:47 -0600
From: Tony Henwood <AnthonyH@chw.edu.au>
Subject: Cromogen/Substrate IHC question -Reply
Katie,
Experience shows that the optimal titre for one ABC technique should be
pretty close for another. The best way is to run the antibodies on your
positive controls as well as tissues you know should be negative.
Positive staining should be similar, but you might find an increase in
background staining. In this case you will need to retitre the localising
antibody and if background is still there then you might have to revert
back to old demonstration technique, try a different demo technique or
put up with the increased background.
Just some thoughts
Tony
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital Westmead,
Locked Bag 4001, Westmead, NSW 2145.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
>>> "Bennett, Catherine (Katie)" <cbennett@lrri.org> 7/February/2001
07:23am >>>
Advice needed:
If I switch an IHC procedure over from an ABC-HRP kit to an ABC-Alk
Phos
kit, I shouldn't have to adjust my primary and secondary antibody
concentrations, right? (I want to change cromogen labels from DAB to
Vector-Red.)
TIA.
*********************************
Catherine "Katie" Bresee Bennett
Sr. Research Technologist
Lovelace Respiratory Research Institute
Albuquerque, New Mexico
----------------------------------------------------------------------
Date: 6 Feb 2001 18:30:03 -0600
From: rnewlin@burnham-inst.org
Subject: confocal
Dear all,
We are offering our confocal laser scanning confocal microscope LSM-410
(Zeiss),
equipped with:
1. internal 543 nm and external 488 and 514 nm lasers
2. Great set of optics ( air, water, glycerol, and oil immersion lenses)
3. New Pentium computer.
If you are interested in this instrument let me know.
----------------------------------------------------------------------
Date: 6 Feb 2001 18:30:40 -0600
From: Steven J Young <stevyoung@juno.com>
Subject: Re: Shurmount
Hi Nancy, You might want to check out IMEBINC.com for mounting media.
They have Solvent 100 which is a mountant that works well with glass
coverslippers and is in stock!!!
On Tue, 06 Feb 2001 14:30:15 -0500 (EST) NLOUISEA@aol.com writes:
> Hello
> We are unable to get Shurmount from our current supplier. Does
> anyone know
> of a source that has Shurmount in stock? and/or what other mounting
> media
> works as well on a Hacker coverslipper?
> Thanks
> Nancy
>
----------------------------------------------------------------------
Date: 6 Feb 2001 18:31:19 -0600
From: DDittus787@aol.com
Subject: Re: xylene distilling
Dear nancy
We have two CBG Recyclers, one for Xylene, the other for alcohol, and are
very happy with service and quality, very little to no maintenance and no
problems. This has enabled us to cut medical waste disposal in 6 mos by 34%
.
dana
******************* NOTE *******************
There may be important message content
contained in the following MIME Information.
********************************************
- ------------------ MIME Information follows ------------------
- --part1_57.113e214b.27b1e152_boundary
Content-Type: text/plain; charset="US-ASCII"
Content-Transfer-Encoding: 7bit
<<<<<< See above "Message Body" >>>>>>
- --part1_57.113e214b.27b1e152_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit
<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>Dear nancy
<BR>We have two CBG Recyclers, one for Xylene, the other for alcohol, and
are
<BR>very happy with service and quality, very little to no maintenance and
no
<BR>problems. This has enabled us to cut medical waste disposal in 6 mos by
34% .
<BR>
&nbs
p; &n
bsp;
dana</FONT></HTML>
- --part1_57.113e214b.27b1e152_boundary--
----------------------------------------------------------------------
Date: 6 Feb 2001 20:15:48 -0600
From: Kathy Gorham <kathyg@eoni.com>
Subject: PG in lab
Good morning fellow histotechs,
Another few questions from Eastern Oregon. Do you have a protocol for
pregnant histotechs? ? ? Are they allowed to continue to work as usual?
The lab manager just presented me with an article that xylene is a risk to
the unborn child. (like I don't know how dangerous our chemicals are) And
wants me to investigate replacements for xylene. I tried several
substitutes years ago and went back to xylene. Do you use a xylene
substitute? Which one? How do you handle the concern for the techs who
are in their child bearing years if you use xylene? Thanks for your
expertise once again.
Kathy Gorham, H.T.
Grande Ronde Hospital
Ls Grande, Oregon
----------------------------------------------------------------------
Date: 6 Feb 2001 20:31:01 -0600
From: Snobird75@aol.com
Subject: Re: xylene distilling
Hello
I have been using the countertop 1 gallon recycler CBG and I really like it.
There are no odors, very easy to use.
I recycle Alcohol and Xylene, the running time is usually 2.5 hrs to 3 hrs.
I have never smelled any odors and I am very happy with the amount of return
of solutions. Also this has greatly cut back on my chemical hazardous
waste.I
now order less Alcohol and xylene.
I have seen no problems with using the recycled solutions on the automatic
stainer.
Although I do still use 100% ALC on the last 2 containers before xylene and
I
do use pure xylene on the last container on the stainer. I feel I could use
all recycled on all the stations and no problems, but my boss said not to
,so
that is the only reason I use pure solutions on those stations.
When I first received the recycler I did have a few problems to work out and
once in a while I still have a few question. The company have been really
great to deal with.
Our lab purchased the one gallon size because we are a research lab.
I wish you well and good luck
Sandi Miller HT
USAMRICD
Maryland
******************* NOTE *******************
There may be important message content
contained in the following MIME Information.
********************************************
- ------------------ MIME Information follows ------------------
- --part1_18.87fed10.27b20b3c_boundary
Content-Type: text/plain; charset="US-ASCII"
Content-Transfer-Encoding: 7bit
<<<<<< See above "Message Body" >>>>>>
- --part1_18.87fed10.27b20b3c_boundary
Content-Type: text/html; charset="US-ASCII"
Content-Transfer-Encoding: 7bit
<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>Hello
<BR>I have been using the countertop 1 gallon recycler CBG and I really like
it.
<BR>There are no odors, very easy to use.
<BR>I recycle Alcohol and Xylene, the running time is usually 2.5 hrs to 3
hrs.
<BR>I have never smelled any odors and I am very happy with the amount of
return
<BR>of solutions. Also this has greatly cut back on my chemical hazardous
waste.I
<BR>now order less Alcohol and xylene.
<BR>I have seen no problems with using the recycled solutions on the
automatic
<BR>stainer.
<BR>Although I do still use 100% ALC on the last 2 containers before xylene
and I
<BR>do use pure xylene on the last container on the stainer. I feel I could
use
<BR>all recycled on all the stations and no problems, but my boss said not
to
,so
<BR>that is the only reason I use pure solutions on those stations.
<BR>When I first received the recycler I did have a few problems to work out
and
<BR>once in a while I still have a few question. The company have been
really
<BR>great to deal with.
<BR>Our lab purchased the one gallon size because we are a research lab.
<BR>I wish you well and good luck
<BR>Sandi Miller HT
<BR>USAMRICD
<BR>Maryland</FONT></HTML>
- --part1_18.87fed10.27b20b3c_boundary--
----------------------------------------------------------------------
Date: 6 Feb 2001 22:45:56 -0600
From: David Taylor Manager <DTMan@KINGMOWER.COM.AU>
Subject: Xylene usage
Dear fellow microtomist's,
If I, a member of my family, close friend, enemy or other was having a
specimen processed on a tissue processor, I would expect fresh reagents. Do
use an enclosed processor if the money is there, do rotate like reagent from
from old to new, do buy a TP that makes this easy, (VIP, or my personel
choice, Leica TP1050.)
We at K&M, and I'm sure others, can tell on opening a retort lid, if the wax
should have been changed yesterday, or on embedding if the fixation was not
optimal.
In pathology, we receive money from patient's for a service provided.
Provide a good service, change reagents, it does make a difference to the
end product.
David.
David Taylor
Laboratory Manager
Drs King & Mower
Adelaide, Australia
Here are the messages received yesterday!
<< Previous Message | Next Message >>