Masson Trichrome on methacrylate sections


I need a procedure for staining Methacrylate sections using Masson Trichrome.
Would it be the same for Paraffin embedded sections, or is there some modification needed?

I also have a Doctor who likes Toluidine Blue O stain for Calcified 5 Micron Bone Sections. I was wondering if there is a better or more effective procedure I can experiment with? 

Diane Beranek
Colorado State University

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