Hairy Cell Antibody- Summary-8 responses
| From: | Daniel & Linda Botsford <dl.botsford@sympatico.ca> |
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Thank you very much for your comments,
We are using:
DBA.44 from Dako catalog # M880. No pretreatment. Primary antibody
dilution of 1:15 for 2 hours at RT, detection on the Ventana using the
basic DAB kit. TRAP from Zymed catalog # 18-0199. HIER in citrate buffer
pH 6.0. Primary antibody dilution of 1:100 for 1 hour at RT, detection
on the Ventana using the basic DAB kit.
DBA 44 dose not work on NexES. If they do Antigen Retrieval and
incubate off line 30 min 1:25 -1:50 it works ok.
DAKO does carry a Hairy Cell Leukemia marker (M0880). We have not
tested it on the NeXES, but it should work in theory. Please contact
DAKO
We use the DBA.44 clone from Dako with a citrate buffer pretreatment
at a dilution of 1:50. I do not know about using on the Nexes, as I have
no experience with that instrument.
The only antibody of which I am aware for Hairy Cell Leukemia is is
DBA-44 but I do not recommend it because it is difficult to work with.
Zymed offers a Ms anti-TRAcP antibody that is useful for identifying
hairy cells (18-0199, clone: ZY-9C5). We recommend epitope retrieval
with citrate buffer, pH 6. We have not tested it at elevated
temperatures. Our distributor in Canada is Intermedico.
Zymed carries a Trap Antiboody for hairy cell leukemia that works very
nicely, microwave HIER and 32 min incubation.
I have trid to get this antibody to work on our NexES but with no real
luck. Zymed makes an anti -TRAcP for hairy cell to be used on
paraffin - never succeded in getting it to work.
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Thank you very much for your comments,
<p>We are using:
<br>DBA.44 from Dako catalog # M880. No pretreatment. Primary antibody
<br>dilution of 1:15 for 2 hours at RT, detection on the Ventana using
the basic DAB kit. TRAP from Zymed catalog # 18-0199. HIER in citrate buffer
pH 6.0. Primary antibody dilution of 1:100 for 1 hour at RT, detection
on the Ventana using the basic DAB kit.
<p> <font color="#3333FF">DBA 44 dose not work on NexES. If
they do Antigen Retrieval and incubate off line 30 min 1:25 -1:50 it works
ok.</font>
<p>DAKO does carry a Hairy Cell Leukemia marker (M0880). We have
not
<br>tested it on the NeXES, but it should work in theory. Please
contact DAKO
<p><font color="#3333FF">We use the DBA.44 clone from Dako with a citrate
buffer pretreatment</font>
<br><font color="#3333FF">at a dilution of 1:50. I do not know about using
on the Nexes, as I have no experience with that instrument.</font>
<p>The only antibody of which I am aware for Hairy Cell Leukemia is is
<br>DBA-44 but I do not recommend it because it is difficult to work with.
<p><font color="#3333FF">Zymed offers a Ms anti-TRAcP antibody that is
useful for identifying</font>
<br><font color="#3333FF">hairy cells (18-0199, clone: ZY-9C5). We recommend
epitope retrieval with citrate buffer, pH 6. We have not tested it at elevated
temperatures. Our distributor in Canada is Intermedico.</font>
<p>Zymed carries a Trap Antiboody for hairy cell leukemia that works very
<br>nicely, microwave HIER and 32 min incubation.
<p><font color="#3333FF">I have trid to get this antibody to work on our
NexES but with no real</font>
<br><font color="#3333FF">luck. Zymed makes an anti -TRAcP
for hairy cell to be used on</font>
<br><font color="#3333FF">paraffin - never succeded in getting it to work.</font></html>
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