Re: Frozen section adherence again!!!!!!!
|From:||Margaret Gondo <firstname.lastname@example.org>|
Commercially available slides can be expensive. There are protocols to
Aminosilane coat your own slides. If you can get your hands on a copy
of "Theory and Practice of Histological Techniques' by Bancroft and
Stevens, there's a nice protocol for doing this on page 500. Coating
your own slides is not that hard and MUCH cheaper than buying them.
Rajan S. Bawa wrote:
> Dear Csaba & Histonetters:
> We have found that for tissues and procedures (including
> immunohistochemistry) that present a big challenge in staying adhered to
> the slide, using aminosilane coated slides helps much more than even
> polylysine slides. Newcomer Supply (800)383-7799 provides these strongly
> adhering slides which come in a variety of colors. Csaba, perhaps these
> could work for you!
> Dr. Rajan Bawa
> Colorado Histoprep
> ----- Original Message -----
> From: Szigeti Csaba <email@example.com>
> To: <firstname.lastname@example.org>
> Sent: Friday, February 09, 2001 3:06 AM
> Subject: Frozen section adherence again!!!!!!!
> > Please write me some tips....
> > my spinal cord slices goes down from the silan slides during the protokoll
> > of in situ hibr.
> > I wrote some days ago but not any answer yet......
> > Csaba
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