Dear Tina  I think that you are dead in the water .. this method as I have
done it in the past needs you to perfuse the animal with a solution
whose contents escape me......the memory is of a sulphide type solution
which smells, the Brain is then removed  it is stained already by the
perfusion solution so only requires cryostat cutting and mounting I seem
to remember that it stains Zinc  but this may be rubbish...however i do
not remember it being particularly difficult  I have proper recipes if you
want to email me direct  hope this helps Mary

On Mon, 28 Feb 2000, Tina Cardamone wrote:

> 
> 
> Hello everybody,
> 
> Is anybody familiar with Timm's method for hippocampal mossy
> fibre sprouting. I need a protocol and all the little bits of advice and 
> tricks that help this method work.
> I have formalin fixed paraffin embeded tissue and I am concerned that fresh 
> tissue is required for this stain!!!!
> 
> Any help and advice will be greatly appreciated.
> 
> I thank you in advance.
> 
> Tina Cardamone
> Pathology Department
> University of Melbourne
> Australia