Ron,

If you are using the Biogenex IHC stainer try the following for CD3 and 
ki-67-------for cd3 we use the Dako concentrate 1:300, Dako citrate 25 min.,
power block 5 min., avidin/biotin block 15 min. ea., primary for 25 min.,
link-20 min., hrp-label 20 min., aec 5 min. (the code # for cd3 from dako is
A0452)-----use tonsil control.   For ki-67, we use Immunotech's primary @
1:100, tonsil control, dako citrate 25 min., power block 5 min., primary for
25 min., link 20 min., hrp-label 20 min., aec 3 min.-----cat. # for the
ki-67 primary is PN IM0505.  Hope this helps. 

> -----Original Message-----
> From:	Pathrm35@cs.com [SMTP:Pathrm35@cs.com]
> Sent:	Friday, February 25, 2000 2:13 PM
> To:	Histonet@pathology.swmed.edu
> Subject:	ki-67 and cd3 immunos
> 
> Fellow Histonetters,
>    I am having problems with CD3 and Ki-67 immunos staining correctly. I
> am 
> using 
> Biogenex antibodies and have tried their procedure by hand and on their 
> immunostainer.
>    I have had mixed results with the Ki-67 and no results with the CD3,
> both 
> on the stainer and by hand. If anyone has any suggestions I greatly 
> appreciate it.
> 
>    Ron Martin, B.Sc. HTL,HT (ASCP)
>    Palm Beach Pathology
>    West Palm Beach, Fl