RE: dehydrated brain...
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From: | Pam Marcum <pmarcum@polysciences.com> |
To: | a i d a n s c h u r r <Aidan.Schurr@hvh.co.nz>, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
Unfortunately, once the complete dehydration is done there is very little
you can do. If it was NBF there is even a good chance you have precipitated
buffer salts all over the outside as a crust. This will further inhibit any
penetration of fluids. Maybe someone else can add more. Pam
-----Original Message-----
From: a i d a n s c h u r r [mailto:Aidan.Schurr@hvh.co.nz]
Sent: Monday, February 21, 2000 11:10 PM
To: histonet@pathology.swmed.edu
Subject: dehydrated brain...
Hi all,
An interesting question / problem...
We have a brain which was fixed approx. 2 years ago in NBF, then
stored. Unfortunately the container was not sealed, and the NBF
evaporated. We now have an extremely dehydrated and shrivelled
brain, and a consultant who wants histology on it!! Any ideas as to
rehydration / salvage would be greatly appreciated.
Cheers,
Aidan Schurr, New Zealand
___________________________________________________
a i d a n c s c h u r r
mlso, histology department
hutt valley health
lower hutt, new zealand
ph. ++64 4 5709173
fax ++64 4 5709214
___________________________________________________
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