Hi Cynthia,

Thanks for taking the time to answer my query.


	Where is the background - just generalized throughtout the section
or in specific cells? -

 I am working on muscle biopsies - so there are few other cells to compare
to - but the nuclei are clean.

 I would not hesitate to use 3%BSA as a protein block
as well as blocking for endogenous peroxidase. I have also found that
background can be decreased by monitoring the substrate time and
concentration as well.
I am optimising  concentration -  the background is consistent throughout
the dilution ranges.

Bottom line is the signal to noise ratio and how
perfect you would like it to be. I like perfection but am unable to attain
it in every stain.
ditto


Louise taylor
Cynthia Favara
NIAID/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
PH: 406-363-9317
FAX: 406-363-9286