RE: CD10

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From:Hewlett Bryan <HEWLETT@HHSC.CA>, 'Richard Cartun' <>


We use Novocastra CD10, clone 56C6(NCL-CD10-270), 1:80 following HIER @ 98C
in citrate buffer pH6.0 for 20 min. For FFPE sections, incubation is
overnight @RT, for B5 or AZF fixed sections, 1 hour @ RT. Detection is by
LSAB, chromogen AEC. With detection by EnVision, dilution can be pushed to
1:160. This antibody works extremely well for us, even after
We performed our initial titrations on sections of normal kidney, small
bowel, tonsil and bone marrow, with the fixatives above, both with and
without demineralization.  We found very little immunoreactivity in normal
tonsil and marrow, what there was being of low intensity. A full range of
intensity was seen in kidney and bowel, we settled on kidney as a procedural
control for CD10. Further validation studies on neoplastic lymphoid lesions,
confirmed our dilutions etc.  We do see very intense reactions in many ALL
cases, we consider this to be the optimum. We have seen no false positives,
as confirmed by flow cytometry, occasional false negs, usually on specimens
that have been rushed through, but thats NO surprise!!!

Best Regards,


> ----------
> From: 	Richard Cartun[]
> Sent: 	February 16, 2000 1:23 PM
> To:
> Subject: 	CD10
> Which antibody are people using for detecting CD10 in formalin-fixed,
> paraffin-embedded tissue?  Thanks!

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