Re: Processing protocol for breast specimens
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From: | RSRICHMOND@aol.com |
To: | histonet@pathology.swmed.edu |
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In a message dated 2/7/00 9:30:38 PM, Allison_Scott@hchd.tmc.edu writes:
<< Our first problem is that the tissues were cut in to big and thick. Our
residents don't listen to reason or follow instructions very well. So
therefore we have to work with some very big fatty pieces of tissue. We are
putting the cassettes into Rapid
Fixx solution(fixative for fatty tisue), for overnight fixation. The next
day, they are put into methanol for 2 to 3 hours. After the methanol, we put
them into acetone for the remainder of the day. >>
You KNOW what the grumpy old pathologist is going to say. I've never seen -
in maybe forty pathology services - such slow and complicated processing of
breast tissue. Your residents are being done no favor by being allowed to cut
so badly - when they get jobs, they won't be nearly so indulged!
Bob Richmond
Samurai Pathologist
Knoxville TN
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