Re: Air Bubbles
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From: | amos brooks <atbrooks@snet.net> |
To: | Jill Songer <jtsonger@vt.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Hi,
If its any consolation I have the same relation with our SAKURA TISSUETEK
coverslippers. The grass aint any greener on this side.
Amos Brooks
Jill Songer wrote:
> I am sooo glad we are not the only ones with a love/hate (mostly hate?)
> relationship with our Hacker coverslipper!
>
> At 09:06 AM 2/7/2000 -0800, Carson, Karla wrote:
> >Tony, We are having the same problems (part of the time) with our Hacker
> >coverslipprer. We think we have it fixed and then it pops up its ugly head
> >again. Most of the time I would like to throw it away and go back to hand
> >coverslipping. If you get any good solutions let us know.
> >Karla Carson HT/HTL (ASCP)
> >Regional Pathology Supervisor
> >Mercy Health Care Sacramento
> >e-mail kcarson@chw.edu <mailto:kcarson@chw.edu>
> >
> > -----Original Message-----
> > From: Tony Henwood [mailto:henwood@mail.one.net.au]
> > Sent: Monday, February 07, 2000 4:17 PM
> > To: histonet@pathology.swmed.edu; Jazz1522@aol.com
> > Subject: Re: Air Bubbles
> >
> > Dear Jazz,
> >
> > > I would like to describe a problem that we have been
> >experiencing in our lab.
> > > The problem that we are experiencing is with our Leica
> >CV5000
> > > (auto-coverslipper):
> > >
> > > * No bubbles are seen in slides after coverslip.
> > >
> > > * After a day or two, a huge air bubble (gap) is formed,
> >surrounding the
> > > tissue specimen.
> > >
> > > * We have increased the volume of the mounting media, but
> >this causes an
> > > overflow of media on top of the cover glass.
> > >
> > > Question: What is your current use of mounting media, and
> >your use of
> > > clearing agent for your staining?
> > >
> > Could it be that the mountant media contains too much
> >xylene?
> >
> > Regards, Tony
> > >
> > Tony Henwood
> > Senior Scientist
> > Anatomical Pathology
> > Royal Prince Alfred Hospital
> > Sydney, AUSTRALIA
> >
> > http://www2.one.net.au/~henwood
> > http://www.pathsearch.com/homepages/TonyHenwood/default.html
> >
> >
>
> **************************************************************************
> Jill Songer HT (ASCP)
> Anatomic Pathology Supervisor
> Virginia Tech
> Veterinary Medical Teaching Hospital
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