Re: Air Bubbles

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From:amos brooks <atbrooks@snet.net>
To:Jill Songer <jtsonger@vt.edu>
Reply-To:
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Hi,
    If its any consolation I have the same relation with our SAKURA TISSUETEK
coverslippers. The grass aint any greener on this side.
Amos Brooks

Jill Songer wrote:

> I am sooo glad we are not the only ones with a love/hate (mostly hate?)
> relationship with our Hacker coverslipper!
>
> At 09:06 AM 2/7/2000 -0800, Carson, Karla wrote:
> >Tony,  We are having the same problems (part of the time) with our Hacker
> >coverslipprer.  We think we have it fixed and then it pops up its ugly head
> >again.  Most of the time I would like to throw it away and go back to hand
> >coverslipping.  If you get any good solutions let us know.
> >Karla Carson HT/HTL (ASCP)
> >Regional Pathology Supervisor
> >Mercy Health Care Sacramento
> >e-mail kcarson@chw.edu <mailto:kcarson@chw.edu>
> >
> >               -----Original Message-----
> >               From:   Tony Henwood [mailto:henwood@mail.one.net.au]
> >               Sent:   Monday, February 07, 2000 4:17 PM
> >               To:     histonet@pathology.swmed.edu; Jazz1522@aol.com
> >               Subject:        Re: Air Bubbles
> >
> >               Dear Jazz,
> >
> >               > I would like to describe a problem that we have been
> >experiencing in our lab.
> >               >  The problem that we are experiencing is with our Leica
> >CV5000
> >               > (auto-coverslipper):
> >               >
> >               > *  No bubbles are seen in slides after coverslip.
> >               >
> >               > *  After a day or two, a huge air bubble (gap) is formed,
> >surrounding the
> >               > tissue specimen.
> >               >
> >               > *  We have increased the volume of the mounting media, but
> >this causes an
> >               > overflow of media on top of the cover glass.
> >               >
> >               > Question:  What is your current use of mounting media, and
> >your use of
> >               > clearing agent for your staining?
> >               >
> >               Could it be that the mountant media contains too much
> >xylene?
> >
> >               Regards, Tony
> >               >
> >               Tony Henwood
> >               Senior Scientist
> >               Anatomical Pathology
> >               Royal Prince Alfred Hospital
> >               Sydney, AUSTRALIA
> >
> >               http://www2.one.net.au/~henwood
> >               http://www.pathsearch.com/homepages/TonyHenwood/default.html
> >
> >
>
> **************************************************************************
> Jill Songer HT (ASCP)
> Anatomic Pathology Supervisor
> Virginia Tech
> Veterinary Medical Teaching Hospital




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