We are also using the Leica CV5000.  We occasionally experience this.  I
think the problem is that the slides are too wet during coverslipping.  When
the clearant evaporates you are left with air bubbles.  The solution is not
to increase the mounting media but to decrease the excess clearant on your
slides.  Make sure to adequately drain the slides before you put them on the
coverslipper.  If you have any questions please feel free to contact me.  We
have had our coverslipper since 4/96.

Jennifer MacDonald

> ----------
> From: 	Tony Henwood[SMTP:henwood@mail.one.net.au]
> Sent: 	Monday, February 07, 2000 6:17 PM
> To: 	histonet@pathology.swmed.edu; Jazz1522@aol.com
> Subject: 	Re: Air Bubbles
> 
> Dear Jazz,
> 
> > I would like to describe a problem that we have been experiencing in our
> lab. 
> >  The problem that we are experiencing is with our Leica CV5000 
> > (auto-coverslipper):
> > 
> > *  No bubbles are seen in slides after coverslip.
> > 
> > *  After a day or two, a huge air bubble (gap) is formed, surrounding
> the 
> > tissue specimen.
> > 
> > *  We have increased the volume of the mounting media, but this causes
> an 
> > overflow of media on top of the cover glass.
> > 
> > Question:  What is your current use of mounting media, and your use of 
> > clearing agent for your staining?
> > 
> Could it be that the mountant media contains too much xylene?
> 
> Regards, Tony 
> > 
> Tony Henwood
> Senior Scientist
> Anatomical Pathology
> Royal Prince Alfred Hospital
> Sydney, AUSTRALIA
> 
> http://www2.one.net.au/~henwood
> http://www.pathsearch.com/homepages/TonyHenwood/default.html
>